Utrera V, Mazzali de Ilja R, Gorziglia M, Esparza J
Res Vet Sci. 1984 May;36(3):310-5.
The prevalence of porcine rotavirus infection in Venezuela was studied from November 1980 to November 1981. Rotaviruses were identified in 21.3 per cent of 286 diarrhoeic samples analysed by electron microscopy and in none of the 48 controls. Rotaviruses were readily detected throughout the year, which may correspond to the absence of seasonal climatic variations in a tropical country such as Venezuela. Rotavirus infection was frequently observed in pigs that were two to six weeks old and was rare in younger animals. The age distribution of rotavirus infection correlated well with the presence of circulating antibodies, as measured by the complement fixation test. Sucking pigs less than two weeks old appeared to be protected from the infection by passive immunity; no antibodies were detected in the two- to three-week-old group, coinciding with an increased susceptibility to rotavirus infection. The antigenic specificity of the rotavirus isolates was confirmed by enzyme-linked immunosorbent assay test, using antibodies directed against the group-specific antigen of human rotavirus. Polyacrylamide gel electrophoresis of viral double-stranded RNA revealed an heterogeneity on the electropherotype of selected samples obtained at different times during the study period.
1980年11月至1981年11月，对委内瑞拉猪轮状病毒感染的流行情况进行了研究。通过电子显微镜分析，在286份腹泻样本中有21.3%检测到轮状病毒，而48份对照样本中均未检测到。全年均可轻易检测到轮状病毒，这可能与委内瑞拉这样的热带国家不存在季节性气候变化有关。轮状病毒感染在2至6周龄的猪中较为常见，在幼龄动物中则很少见。轮状病毒感染的年龄分布与通过补体结合试验测定的循环抗体的存在情况密切相关。不到两周龄的吮乳仔猪似乎通过被动免疫免受感染；在2至3周龄组中未检测到抗体，这与对轮状病毒感染的易感性增加相一致。使用针对人轮状病毒群特异性抗原的抗体，通过酶联免疫吸附试验证实了轮状病毒分离株的抗原特异性。病毒双链RNA的聚丙烯酰胺凝胶电泳显示，在研究期间不同时间获得的选定样本的电泳图谱存在异质性。
