Kariniemi A L, Lehto V P, Virtanen I
Br J Dermatol. 1984 Aug;111(2):139-45. doi: 10.1111/j.1365-2133.1984.tb04036.x.
We studied the localization of p230, an immunoanalogue of erythroid alpha-spectrin, in normal and psoriatic human epidermis and in cultured human keratinocytes. In immunofluorescence microscopy of frozen sections of normal skin a bright cytoplasmic staining was seen in the cortical area of the keratinocytes. Similar staining was also seen in lesional and uninvolved areas of psoriatic epidermis. The pericytoplasmic localization of p230 could also be seen in cultured human keratinocytes: a lamina-like reticular staining was seen mostly confined to the ventral cytoplasmic aspect and to junctional areas of the cells. Immunoblotting of electrophoretically separated polypeptides of epidermal cells revealed a distinct polypeptide of Mr 230 kD. The results indicate that alpha-spectrin-like polypeptides form a major cytoskeletal framework in human epidermal cells in both normal and psoriatic skin.
我们研究了红细胞α-血影蛋白的免疫类似物p230在正常和银屑病患者的人类表皮以及培养的人类角质形成细胞中的定位。在正常皮肤冰冻切片的免疫荧光显微镜检查中，在角质形成细胞的皮质区域可见明亮的细胞质染色。在银屑病表皮的病变区和非病变区也观察到类似的染色。p230在细胞质周围的定位在培养的人类角质形成细胞中也可见：可见一种片状网状染色，主要局限于细胞的腹侧细胞质部分和连接区域。对表皮细胞经电泳分离的多肽进行免疫印迹分析，显示出一条明显的230 kD的多肽。结果表明，α-血影蛋白样多肽在正常和银屑病皮肤的人类表皮细胞中形成主要的细胞骨架框架。
