Nordvall S L, Uhlin T, Einarsson R, Johansson S G, Ohman S
Clin Allergy. 1984 Jul;14(4):341-50. doi: 10.1111/j.1365-2222.1984.tb02214.x.
The immune response to honey bee venom in thirty-seven bee keepers' sera was studied by several methods. Specific IgE antibody levels studied by RAST were generally low, whereas specific IgG antibody levels studied by a Sepharose protein A technique were high. Crossed radioimmunoelectrophoresis was applied for a detailed analysis of the antibody specificities towards the different components of venom in seventeen of the bee keepers' sera. Significant amounts of IgG antibodies were found towards most bee-venom components. The highest IgG response was directed towards phospholipase A. Hyaluronidase, acid phosphatase and two uncharacterized antigens also showed distinct IgG binding. The IgG binding to melittin was low. The IgE binding to the bee venom components was low and primarily directed to the phospholipase. IgE binding to hyaluronidase and acid phosphatase occurred, but was also in very small amounts. One bee-keeper serum caused heavy radiostaining to melittin but the others did not show IgE binding to this component. Thus a low IgE but a high IgG response was demonstrated in bee keepers. The major immunogen was phospholipase A, which is known to be the major allergen in bee venom. Generally, the strongest IgG responses were found to the components capable of inducing the strongest IgE responses.
通过多种方法研究了37名养蜂人血清对蜂毒的免疫反应。用放射变应原吸附试验（RAST）研究的特异性IgE抗体水平普遍较低，而用琼脂糖蛋白A技术研究的特异性IgG抗体水平较高。应用交叉放射免疫电泳对17名养蜂人血清中针对蜂毒不同成分的抗体特异性进行详细分析。发现针对大多数蜂毒成分都有大量的IgG抗体。最高的IgG反应针对磷脂酶A。透明质酸酶、酸性磷酸酶和两种未鉴定的抗原也显示出明显的IgG结合。IgG与蜂毒肽的结合较低。IgE与蜂毒成分的结合较低，主要针对磷脂酶。IgE与透明质酸酶和酸性磷酸酶的结合也有发生，但量也非常少。一份养蜂人血清对蜂毒肽产生强烈的放射染色，但其他血清未显示出IgE与该成分的结合。因此，养蜂人表现出低IgE但高IgG反应。主要免疫原是磷脂酶A，已知它是蜂毒中的主要变应原。一般来说，对能够诱导最强IgE反应的成分发现了最强的IgG反应。
