Papsidero L D, Croghan G A, Wang M C, Kuriyama M, Johnson E A, Valenzuela L A, Chu T M
Hybridoma. 1983;2(2):139-47. doi: 10.1089/hyb.1983.2.139.
Hybridoma culture F5 has been developed which secretes monoclonal antibody (McAb) directed to an epitope of a prostatic glycoprotein of Mr 34 kD (Prostate Antigen, PA). Tissue levels of PA have been evaluated using a competitive-binding enzyme-immunoassay based upon the inhibition of McAb binding activity to purified antigen. Results indicated the specific occurrence of high antigen concentrations in extracts prepared from prostatic tissues. The antigenicity of epitope F5 is resistant to tissue fixation and embedding protocols, and has been demonstrated upon immunoperoxidase staining procedures. Immunoperoxidase data strongly indicate that McAb F5 possesses a singular specificity towards prostatic epithelial cells. Other tissues, whether normal or cancerous, fail to express this determinant. Specimens examined included epithelial and nonepithelial tissues along with a panel of carcinomas and sarcomas. The antibody was able to detect tumor cells at extra-prostatic sites and represents a powerful probe for the detection and differential diagnosis of metastatic cancer of the prostate.
已培养出杂交瘤F5，它分泌针对分子量为34kD的前列腺糖蛋白（前列腺抗原，PA）一个表位的单克隆抗体（McAb）。基于McAb与纯化抗原结合活性的抑制作用，采用竞争性结合酶免疫测定法评估了PA的组织水平。结果表明，从前列腺组织制备的提取物中特异性地出现高抗原浓度。表位F5的抗原性对组织固定和包埋方案具有抗性，并已在免疫过氧化物酶染色程序中得到证实。免疫过氧化物酶数据有力地表明，McAb F5对前列腺上皮细胞具有独特的特异性。其他组织，无论是正常组织还是癌组织，均不表达这种决定簇。检查的标本包括上皮和非上皮组织以及一组癌和肉瘤。该抗体能够检测前列腺外部位的肿瘤细胞，是检测和鉴别诊断前列腺转移性癌的有力探针。
