Chu T M, Kawinski E, Hibi N, Croghan G, Wiley J, Killian C S, Corral D
Department of Diagnostic Immunology Research, Roswell Park Memorial Institute, Buffalo, NY 14263.
J Urol. 1989 Jan;141(1):152-6. doi: 10.1016/s0022-5347(17)40630-6.
With the use of five murine monoclonal antibodies (1A5, 2A4, 3F1, F5 and 3A12) and an antigen-affinity purified goat polyclonal IgG antibody, the presence of a prostate-specific antigenic domain in human prostate-specific antigen molecule was identified. The results were based upon a series of quantitative competitive inhibition assays of each 125I-labeled monoclonal antibody and polyclonal antibody binding to prostate-specific antigen by unlabeled monoclonal antibodies as inhibitors, and immunohistochemical examination of an extensive panel of human tissue specimens. A cluster of two epitopes that are spatially related or in close topographical proximity and represent a prostate-specific antigenic domain are defined by the monoclonal antibodies 1A5, 2A4, 3F1, and F5, 3A12, respectively.
