Keszthelyi L, Varga L, Demeter I, Hollós-Nagy K, Szókefalvi-Nagy Z
Anal Biochem. 1984 Jun;139(2):418-26. doi: 10.1016/0003-2697(84)90028-9.
The particle-induced X-ray emission (PIXE) method and the 14N(d,p)15N nuclear reaction are combined for simultaneous trace element and nitrogen determination. Measurement of nitrogen content often allows the relating of the elemental concentrations determined by PIXE to the protein content of the sample. For the measurements only a small amount of sample material is needed; therefore, it is possible to keep track of the quantity of a certain element in the successive steps of a biomedical separation process. In about 10 min, trace element concentrations in the ppm range can be determined with a statistical accuracy of about 10%.
将粒子诱导X射线发射（PIXE）方法与14N（d，p）15N核反应相结合，可同时测定微量元素和氮含量。氮含量的测量通常能将PIXE测定的元素浓度与样品中的蛋白质含量联系起来。进行测量仅需少量样品材料；因此，在生物医学分离过程的连续步骤中能够追踪某一特定元素的量。在大约10分钟内，可以测定ppm范围内的微量元素浓度，统计准确度约为10%。
