Elseweidy M M, Fadel H E, Abraham E C
Hemoglobin. 1984;8(4):363-72. doi: 10.3109/03630268408991719.
Cord blood samples were obtained at delivery from women at gestational periods of 30 to 36 weeks (preterm) and from a control group at 40 weeks of gestational age (term). Total glycosylated hemoglobin (GHb) was determined by affinity chromatography and the percentages of the minor Hbs FIa+b and FIc (of total Hb F) were determined by Bio-Rex 70 chromatography in whole blood samples and in red cell populations separated by Dextran 40 density gradient centrifugation. The absolute levels and the increase due to red cell aging of GHb, Hb FIa+b and Hb FIc were significantly reduced in the preterm samples compared to the term samples indicating decreased glycosylation in preterm fetus. When the nonglycosylated Hbs from term and preterm newborns were separated by Bio-Rex 70 chromatography after removal of the GHb by affinity chromatography, the acetylated form of Hb FIc also showed an increase with red cell aging indicating posttranslational enzymatic or nonenzymatic acetylation of Hb F during the entire life span of red cells. Moreover, as with GHb, the acetylated Hb was also decreased in the preterm newborns.
在分娩时从孕周为30至36周的早产女性以及孕周为40周的对照组（足月）女性获取脐血样本。通过亲和色谱法测定总糖化血红蛋白（GHb），并通过Bio-Rex 70色谱法在全血样本以及经葡聚糖40密度梯度离心分离的红细胞群体中测定次要血红蛋白FIa + b和FIc（占总血红蛋白F的百分比）。与足月样本相比，早产样本中GHb、Hb FIa + b和Hb FIc的绝对水平以及因红细胞衰老而增加的量均显著降低，表明早产胎儿的糖基化减少。当通过亲和色谱法去除GHb后，通过Bio-Rex 70色谱法分离足月和早产新生儿的非糖化血红蛋白时，Hb FIc的乙酰化形式也显示随红细胞衰老而增加，表明在红细胞的整个寿命期间Hb F发生了翻译后酶促或非酶促乙酰化。此外，与GHb一样，早产新生儿中的乙酰化血红蛋白也减少。
