Abraham E C, Stallings M, Abraham A, Elseweidy M M
Hemoglobin. 1983;7(5):449-60. doi: 10.3109/03630268309038414.
Levels of glycosylated hemoglobin (glyco Hb) were determined in 45 newborn infants by affinity chromatography on phenylboronate-agarose gel and found to correlate well with the results obtained by two chemical methods. However the percentages of Hb FIa+b and Hb FIc determined by Bio-Rex 70 chromatography did not correlate with the glyco Hb values. The glyco Hb levels in the newborns were significantly lower than in the adults. Rechromatography of the glyco and nonglyco Hb fractions on Bio-Rex 70 columns indicated that Hb FO-like glyco Hb (presumably formed by glycosylation of the alpha chain amino terminus and epsilon-amino groups of lysyl amino acids) constituted about 60% of the glyco Hb fraction, whereas both Hb FIc and Hb FIa+b components contained only small portions of the glyco Hb.
采用苯硼酸 - 琼脂糖凝胶亲和色谱法测定了45例新生儿的糖化血红蛋白（糖基化血红蛋白）水平，发现其与两种化学方法所得结果相关性良好。然而，通过Bio - Rex 70色谱法测定的Hb FIa + b和Hb FIc的百分比与糖基化血红蛋白值不相关。新生儿的糖基化血红蛋白水平显著低于成年人。在Bio - Rex 70柱上对糖化和非糖化血红蛋白组分进行再色谱分析表明，类似Hb FO的糖化血红蛋白（可能由α链氨基末端和赖氨酸氨基酸的ε - 氨基糖基化形成）约占糖化血红蛋白组分的60%，而Hb FIc和Hb FIa + b组分仅含有少量的糖化血红蛋白。
