Hanson J, Coombs A, Moore J L
Int J Radiat Oncol Biol Phys. 1984 Sep;10(9):1697-701. doi: 10.1016/0360-3016(84)90531-5.
Since 1981 we have received 50 tumor samples from 10 different sites; over half were breast or ovary. Of the 27 that were considered suitable for cloning, 11 produced colony formation and 6 of these were drug tested. One ovarian granulosa cell tumor and its xenograft (V7) were tested against several cytotoxic agents. During a period of 16 months, sensitivity to cisplatin was relatively stable but sensitivity to vinblastine was markedly changed when the original tumor cells and original cells stored in liquid nitrogen were compared with xenograft cells. These changes may be related to patient treatments prior to tumor sample collection. This xenograft V7 exhibited chromosome karyotype and iso-enzyme Glucose-6-phosphate dehydrogenase consistent with it being of human origin. Gross histology of original tumor and xenograft were similar. Chemosensitization in vivo of a breast xenograft (Hx99) to melphalan by misonidazole was investigated. Misonidazole at a total dose of 0.5 g/kg given prior to melphalan (14 mg/kg) was an effective chemosensitizer.
自1981年以来，我们从10个不同部位获取了50个肿瘤样本；其中一半以上来自乳腺或卵巢。在被认为适合克隆的27个样本中，11个形成了集落，其中6个进行了药物测试。对1例卵巢颗粒细胞瘤及其异种移植瘤（V7）进行了几种细胞毒性药物的测试。在16个月的时间里，当将原始肿瘤细胞和液氮保存的原始细胞与异种移植细胞进行比较时，对顺铂的敏感性相对稳定，但对长春碱的敏感性发生了显著变化。这些变化可能与肿瘤样本采集前患者接受的治疗有关。该异种移植瘤V7的染色体核型和同工酶葡萄糖-6-磷酸脱氢酶显示其来源于人类。原始肿瘤和异种移植瘤的大体组织学相似。研究了咪索硝唑对乳腺异种移植瘤（Hx99）体内美法仑化疗增敏作用。在给予美法仑（14mg/kg）之前给予总量为0.5g/kg的咪索硝唑是一种有效的化疗增敏剂。
