Rweyemamu M M
J Biol Stand. 1984 Jul;12(3):323-37. doi: 10.1016/s0092-1157(84)80013-x.
The neutralization reaction is the most appropriate in vitro reference test system for assessing intratypic antigenic variation as it involves the antigenic determinants responsible for virus strain specificity and evoking protective antibody. Antigenic relationships determined in different neutralization test systems were independent of the system used and were assumed to truly reflect antigenic variation. The two-dimensional microneutralization test was found to be appropriate for foot and mouth disease (FMD) virus strain differentiation. To minimize test to test variation, comparisons are performed as matched pairs. The pooled variance of the test system is used to assess the significance of the relationships obtained. Antisera from convalescent animals were less specific than those from vaccinates. Serum quality seemed less critical for the virus neutralization than the complement fixation reaction. A system for FMD virus strain differentiation based on the use of the virus neutralization reaction taking into account the statistical and biological significance of observed r values is recommended.
中和反应是评估型内抗原变异最合适的体外参考测试系统，因为它涉及负责病毒株特异性和激发保护性抗体的抗原决定簇。在不同中和测试系统中确定的抗原关系与所使用的系统无关，并被认为能真实反映抗原变异。二维微量中和试验被发现适用于口蹄疫（FMD）病毒株的鉴别。为尽量减少试验间的变异，比较以配对方式进行。测试系统的合并方差用于评估所得关系的显著性。恢复期动物的抗血清不如接种疫苗动物的抗血清特异性强。血清质量对病毒中和反应的重要性似乎低于补体结合反应。推荐一种基于病毒中和反应的口蹄疫病毒株鉴别系统，该系统考虑了观察到的r值的统计学和生物学意义。
