Shanahan F, Lee T D, Bienenstock J, Befus A D
J Allergy Clin Immunol. 1984 Oct;74(4 Pt 1):499-504. doi: 10.1016/0091-6749(84)90385-3.
The histamine-releasing effect of certain opiate drugs prompted a survey of endogenous opiates for mast cell-secretagogue activity. Since intestinal mucosal mast cells (MMC) differ from connective tissue mast cells in their response to a variety of secretagogues and anti-allergic compounds, we have examined the influence of several endogenous opiate peptides on histamine secretion from the two mast cell types in the rat. MMC hyperplasia was induced in rats infected with the nematode Nippostrongylus brasiliensis and MMC were isolated by collagenase digestion from the small intestine. Connective tissue mast cells from the peritoneal cavity (PMC) were isolated by peritoneal lavage. Dynorphin, alpha-neoendorphin, and beta-endorphin had a concentration-dependent secretagogue effect (10(-6)M to 10(-4)M) on PMC that was temperature and energy dependent, but MMC from the same animals were unresponsive to these agents. Differences between PMC and MMC did not appear to be attributable to the MMC isolation procedure since PMC treated similarly remained responsive to endorphin. Endorphin-induced histamine secretion from PMC was partially inhibited by the anti-allergic agent disodium cromoglycate. Inhibition with the opiate antagonist naloxone was nonspecific, occurring only at concentrations that also inhibited antigen-induced mediator release. Mast cell secretion induced by certain opiate peptides may therefore be nonreceptor mediated and relate to a direct membrane effect by basic peptides.
某些阿片类药物的组胺释放作用促使人们对内源性阿片类物质进行肥大细胞促分泌活性的研究。由于肠黏膜肥大细胞（MMC）与结缔组织肥大细胞对多种促分泌剂和抗过敏化合物的反应不同，我们研究了几种内源性阿片肽对大鼠两种肥大细胞组胺分泌的影响。用巴西日圆线虫感染大鼠诱导MMC增生，通过胶原酶消化从小肠分离MMC。通过腹腔灌洗从腹腔分离结缔组织肥大细胞（PMC）。强啡肽、α-新内啡肽和β-内啡肽对PMC具有浓度依赖性促分泌作用（10⁻⁶M至10⁻⁴M），且这种作用依赖于温度和能量，但来自相同动物的MMC对这些物质无反应。PMC和MMC之间的差异似乎并非归因于MMC的分离过程，因为经类似处理的PMC对内啡肽仍有反应。抗过敏剂色甘酸钠可部分抑制内啡肽诱导的PMC组胺分泌。阿片拮抗剂纳洛酮的抑制作用是非特异性的，仅在也能抑制抗原诱导的介质释放的浓度下才会发生。因此，某些阿片肽诱导的肥大细胞分泌可能是非受体介导的，且与碱性肽的直接膜效应有关。
