通过碱性提取十二烷基硫酸钠 - 聚丙烯酰胺平板凝胶中萘酚蓝黑染色的多肽来定量蛋白质。
Quantitation of protein by alkaline extraction of naphthol blue black-stained polypeptides in sodium dodecyl sulfate-polyacrylamide slab-gels.
作者信息
Everitt E, Maksimova A
出版信息
Anal Biochem. 1984 Aug 15;141(1):17-24. doi: 10.1016/0003-2697(84)90419-6.
PMID:6208809
Abstract
A sodium dodecyl sulfate-polyacrylamide slab-gel system was used to study the alkaline extraction of Naphthol Blue Black B-stained polypeptides. A simple, rapid, and reproducible method for quantitative assessment of 1- to 5-micrograms quantities of individual polypeptides in the molecular weight range of 14,000 to 120,000 is described. The working range of the assay may be extended to the 20-micrograms level at the expense of rapidity.
摘要
采用十二烷基硫酸钠-聚丙烯酰胺平板凝胶系统研究了萘酚蓝黑B染色多肽的碱性提取。本文描述了一种简单、快速且可重复的方法，用于定量评估分子量在14,000至120,000范围内1至5微克的单个多肽。该测定的工作范围可以扩展到20微克水平，但会牺牲速度。
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