Clark D A, Garcia N J, Rozell P R, Mosser E L
Aviat Space Environ Med. 1984 Oct;55(10):941-5.
Titration of serum lipoproteins with three different precipitants--heparin-MnCl2 92 mmol.L-1, dextran sulfate-MgSO4, and phosphotungstate-MgCl2--revealed gross errors in the high density lipoprotein cholesterol (HDL-C) levels measured in some hyperlipidemic sera. Dilution studies of eight normal sera with the phosphotungstate method revealed that the most reliable estimate of the HDL-C level was the level measured in the least diluted aliquot that yielded a good precipitate. With a heparin-MnCl2 kit, serial dilution of a lipemic serum demonstrated a requirement for a blank correction. Establishing an accurate estimate of the HDL-C level sometimes requires knowledge of the titration curve of the lipoprotein precipitant being used, a blank correction, and data obtained from dilution studies (or studies with increased precipitant levels) on the serum in question.
用三种不同的沉淀剂——92毫摩尔/升的肝素 - 氯化锰、硫酸葡聚糖 - 硫酸镁和磷钨酸 - 氯化镁对血清脂蛋白进行滴定，结果显示在某些高脂血症血清中测量的高密度脂蛋白胆固醇（HDL - C）水平存在重大误差。用磷钨酸法对八份正常血清进行稀释研究表明，HDL - C水平最可靠的估计值是在产生良好沉淀的最不稀释的等分试样中测得的水平。使用肝素 - 氯化锰试剂盒时，对一份脂血血清进行系列稀释表明需要进行空白校正。要准确估计HDL - C水平，有时需要了解所使用的脂蛋白沉淀剂的滴定曲线、进行空白校正以及从对相关血清的稀释研究（或增加沉淀剂水平的研究）中获得的数据。
