Warnick G R, Nguyen T, Albers A A
Clin Chem. 1985 Feb;31(2):217-22.
We compared the standard Lipid Research Clinics heparin-Mn2+ (46 mmol/L) method and five improved precipitation methods for quantification of high-density lipoprotein (HDL) cholesterol. Three of these methods--a dextran sulfate-Mg2+ procedure, reported as a Selected Method, a modified heparin-Mn2+ (92 mmol/L) method, and a modified phosphotungstate-Mg2+ procedure--all gave similar results. Three other methods--the standard heparin-Mn2+ (46 mmol/L) method and two polyethylene glycol methods (75 g/L or pH 10 reagent at 100 g/L final concentrations)--gave slightly higher values for HDL cholesterol. Addition of NaCl or glucose to specimens did not significantly change protein precipitation. In terms of sedimentation effectiveness with hypertriglyceridemic specimens, the methods were ranked in the following order: polyethylene glycol (pH 10, 100 g/L) greater than dextran sulfate-Mg2+ greater than heparin-Mn2+ (92 mmol/L) = polyethylene glycol (75 g/L) greater than phosphotungstate-Mg2+ greater than heparin-Mn2+ (46 mmol/L).
我们比较了标准的脂质研究诊所肝素-Mn2+（46 mmol/L）法和五种改进的沉淀法用于定量高密度脂蛋白（HDL）胆固醇。其中三种方法——一种硫酸葡聚糖-Mg2+法（报告为选定方法）、一种改良的肝素-Mn2+（92 mmol/L）法和一种改良的磷钨酸-Mg2+法——均给出了相似的结果。另外三种方法——标准的肝素-Mn2+（46 mmol/L）法和两种聚乙二醇法（最终浓度分别为75 g/L或pH 10试剂100 g/L）——给出的HDL胆固醇值略高。向标本中添加NaCl或葡萄糖不会显著改变蛋白质沉淀。就对高甘油三酯血症标本的沉淀效果而言，这些方法的排序如下：聚乙二醇（pH 10，100 g/L）大于硫酸葡聚糖-Mg2+大于肝素-Mn2+（92 mmol/L）=聚乙二醇（75 g/L）大于磷钨酸-Mg2+大于肝素-Mn2+（46 mmol/L）。
