Saurat J H, Didierjean L, Skalli O, Siegenthaler G, Gabbiani G
J Invest Dermatol. 1984 Dec;83(6):431-5. doi: 10.1111/1523-1747.ep12273528.
Four hundred Merkel cells (MC) have been studied by double-label immunofluorescence using: (1) a monoclonal antibody which has been previously demonstrated to react with MC and (2) antisera and monoclonal antibodies against the 5 types of intermediate filaments. It was demonstrated that MC did not react with vimentin, desmin, glial acidic fibrillary protein, or neurofilament antisera. A strong staining of MC was observed with 2 antisera and 2 monoclonal antibodies against keratin. The cytokeratin polypeptide pattern of MC is probably similar to that of simple epithelia. These findings attest to the epithelial nature of MC.
已使用以下方法通过双标记免疫荧光对400个默克尔细胞（MC）进行了研究：（1）一种先前已证明可与MC发生反应的单克隆抗体，以及（2）针对5种中间丝类型的抗血清和单克隆抗体。结果表明，MC不与波形蛋白、结蛋白、胶质酸性纤维蛋白或神经丝抗血清发生反应。用2种抗血清和2种针对角蛋白的单克隆抗体观察到MC有强烈染色。MC的细胞角蛋白多肽模式可能与简单上皮细胞的相似。这些发现证明了MC的上皮性质。
