Tybulewicz V L, Falk G, Walker J E
J Mol Biol. 1984 Oct 25;179(2):185-214. doi: 10.1016/0022-2836(84)90465-0.
The nucleotide sequence has been determined of a 12,368 base-pair region of DNA cloned from the non-sulphur photosynthetic bacterium Rhodopseudomonas blastica. It contains a cluster of six genes of which five encode the subunits of F1-ATPase; the sixth codes for an unknown protein. The genes are arranged in the same order as in the Escherichia coli unc operon, except that the unknown gene is placed between those for gamma and beta subunits. Neither the genes for F0 subunits, nor a homologue of the E. coli uncI gene is associated with this locus. The six genes are transcribed from a single promoter and we have designated this region the R. blastica atp operon. The two distal genes, beta and epsilon, may also be transcribed from a second promoter. Initiation and termination points for transcription have been identified by primer extensions and S1 nuclease mapping experiments. Signals involved in initiation of translation (Shine and Dalgarno sequences) and termination of transcription in the photosynthetic bacterium resemble those in E. coli. However, no common features can be identified in these two bacteria between 5' regions adjacent to sites of initiation of transcription. The sequence also contains a gene that encodes a protein homologous to discoidin, a cell surface lectin of Dictyostelium discoideum thought to be involved in cell--cell aggregation. Seven other reading frames have not been identified.
已确定从非硫光合细菌 Blastica 红假单胞菌克隆的一段 12368 个碱基对的 DNA 区域的核苷酸序列。它包含一组六个基因，其中五个编码 F1 - ATP 酶的亚基；第六个编码一种未知蛋白质。这些基因的排列顺序与大肠杆菌 unc 操纵子中的相同，只是未知基因位于γ和β亚基的基因之间。F0 亚基的基因以及大肠杆菌 uncI 基因的同源物均与该基因座无关。这六个基因从单个启动子转录，我们将该区域命名为 Blastica 红假单胞菌 atp 操纵子。两个远端基因，β和ε，也可能从第二个启动子转录。转录的起始和终止点已通过引物延伸和 S1 核酸酶图谱实验确定。光合细菌中参与翻译起始（Shine 和 Dalgarno 序列）和转录终止的信号与大肠杆菌中的相似。然而，在这两种细菌中，转录起始位点相邻的 5' 区域之间未发现共同特征。该序列还包含一个基因，其编码的蛋白质与盘基网柄菌的盘状菌素同源，盘状菌素是盘基网柄菌的一种细胞表面凝集素，被认为参与细胞间聚集。尚未鉴定出其他七个阅读框。
