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人骨肉瘤细胞中c-sis转录本的检测及血小板衍生生长因子样蛋白的合成

Detection of c-sis transcripts and synthesis of PDGF-like proteins by human osteosarcoma cells.

作者信息

Graves D T, Owen A J, Barth R K, Tempst P, Winoto A, Fors L, Hood L E, Antoniades H N

出版信息

Science. 1984 Nov 23;226(4677):972-4. doi: 10.1126/science.6209798.

DOI:10.1126/science.6209798
PMID:6209798
Abstract

Platelet-derived growth factor (PDGF) has been previously shown to be homologous to the transforming gene of simian sarcoma virus (v-sis), and inappropriate expression of the cellular counterpart of the v-sis gene (c-sis) has been implicated in the generation of mesenchymal tumors. The U-2 OS human osteosarcoma line was shown to contain multiple c-sis transcripts. Immunoprecipitation experiments with antiserum to PDGF identified a variety of polypeptides ranging in size from 18,000 to 165,000 daltons that were immunoprecipitated specifically from U-2 OS cell extracts. The osteosarcoma also was shown to secrete a 29,000-dalton protein having the serological and structural characteristics of PDGF.

摘要

血小板衍生生长因子（PDGF）先前已被证明与猿猴肉瘤病毒（v-sis）的转化基因同源，v-sis基因（c-sis）的细胞对应物的不适当表达与间充质肿瘤的发生有关。已证明U-2 OS人骨肉瘤细胞系含有多种c-sis转录本。用抗PDGF抗血清进行的免疫沉淀实验鉴定出多种大小在18,000至165,000道尔顿之间的多肽，这些多肽是从U-2 OS细胞提取物中特异性免疫沉淀出来的。骨肉瘤还被证明能分泌一种具有PDGF血清学和结构特征的29,000道尔顿蛋白质。

相似文献

1
Detection of c-sis transcripts and synthesis of PDGF-like proteins by human osteosarcoma cells.人骨肉瘤细胞中c-sis转录本的检测及血小板衍生生长因子样蛋白的合成
Science. 1984 Nov 23;226(4677):972-4. doi: 10.1126/science.6209798.
2
[Detection of c-sis transcripts and PDGF-like products by in situ hybridization and immunohistochemical study].[通过原位杂交和免疫组织化学研究检测c-sis转录本和血小板衍生生长因子样产物]
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Human leukemia cells synthesize and secrete proteins related to platelet-derived growth factor.人类白血病细胞合成并分泌与血小板衍生生长因子相关的蛋白质。
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5526-30. doi: 10.1073/pnas.83.15.5526.
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Imbalanced expression of cellular nuclear oncogenes caused by v-sis/PDGF-2.由v-sis/血小板衍生生长因子-2导致的细胞核癌基因表达失衡。
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Kidney epithelial cells express c-sis protooncogene and secrete PDGF-like protein.肾上皮细胞表达c-sis原癌基因并分泌血小板源性生长因子样蛋白。
Am J Physiol. 1988 Oct;255(4 Pt 2):F800-6. doi: 10.1152/ajprenal.1988.255.4.F800.
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A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains.一种人骨肉瘤细胞系分泌一种生长因子，其结构与血小板衍生生长因子A链同二聚体相关。
Nature. 1986;319(6053):511-4. doi: 10.1038/319511a0.
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The human osteosarcoma cell line U-2 OS expresses a 3.8 kilobase mRNA which codes for the sequence of the PDGF-B chain.人骨肉瘤细胞系U-2 OS表达一种3.8千碱基的信使核糖核酸，该信使核糖核酸编码血小板源性生长因子-B链的序列。
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SIS/PDGF-B promoter isolation and characterization of regulatory elements necessary for basal expression of the SIS/PDGF-B gene in U2-OS osteosarcoma cells.SIS/PDGF-B启动子的分离以及U2-OS骨肉瘤细胞中SIS/PDGF-B基因基础表达所需调控元件的鉴定
J Biol Chem. 1994 Nov 18;269(46):28648-54.

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Overexpression of PDGF-BB decreases colorectal and pancreatic cancer growth by increasing tumor pericyte content.血小板衍生生长因子-BB（PDGF-BB）的过表达通过增加肿瘤周细胞含量来降低结直肠癌和胰腺癌的生长。
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Signals controlling the expression of PDGF.控制血小板源性生长因子（PDGF）表达的信号。
Mol Biol Rep. 1995;22(1):1-24. doi: 10.1007/BF00996300.
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A novel human c-sis mRNA species is transcribed from a promoter in c-sis intron 1 and contains the code for an alternative PDGF B-like protein.一种新的人类c-sis mRNA转录本从c-sis内含子1中的一个启动子转录而来，并且包含一种替代性血小板衍生生长因子B样蛋白的编码。
Nucleic Acids Res. 1995 Aug 11;23(15):2815-22. doi: 10.1093/nar/23.15.2815.
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Synthesis and secretion of proteins resembling platelet-derived growth factor by human glioblastoma and fibrosarcoma cells in culture.培养的人胶质母细胞瘤和纤维肉瘤细胞合成与分泌类似血小板衍生生长因子的蛋白质。
Proc Natl Acad Sci U S A. 1985 Apr;82(8):2404-8. doi: 10.1073/pnas.82.8.2404.
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Spontaneous expression of the c-sis gene and release of a platelet-derived growth factorlike molecule by human alveolar macrophages.人肺泡巨噬细胞中c-sis基因的自发表达及血小板衍生生长因子样分子的释放。
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