Pantazis P, Pelicci P G, Dalla-Favera R, Antoniades H N
Proc Natl Acad Sci U S A. 1985 Apr;82(8):2404-8. doi: 10.1073/pnas.82.8.2404.
Immunoprecipitation of proteins extracted from metabolically labeled human glioblastoma and fibrosarcoma cells with antiserum to platelet-derived growth factor (PDGF) showed that these cells express and secrete proteins that are recognized specifically by the antiserum. The molecular masses of immunoprecipitated proteins in the lysates of the malignant cells ranged from 16 kDa to 140 kDa. Both cell lines secreted a 31-kDa polypeptide with structural, immunological, and biological properties similar to those of human PDGF. These cell lines were shown to synthesize a 4.4-kb mRNA that contained sequences from all the six currently identified exons of the human c-sis gene. These data suggest that the PDGF-like proteins in the two mesenchyme-derived transformed cells are encoded at least in part by the c-sis locus.
用抗血小板衍生生长因子（PDGF）抗血清对从经代谢标记的人胶质母细胞瘤和纤维肉瘤细胞中提取的蛋白质进行免疫沉淀，结果表明这些细胞表达并分泌能被该抗血清特异性识别的蛋白质。恶性细胞裂解物中免疫沉淀蛋白质的分子量范围为16 kDa至140 kDa。两种细胞系均分泌一种31 kDa的多肽，其结构、免疫学和生物学特性与人类PDGF相似。这些细胞系被证明能合成一种4.4 kb的mRNA，该mRNA包含人类c-sis基因目前已鉴定的所有六个外显子的序列。这些数据表明，两种间充质来源的转化细胞中的类PDGF蛋白至少部分由c-sis基因座编码。
