Saku T, Okabe H, Yagi Y, Sato E, Tsuda N
Acta Pathol Jpn. 1984 Sep;34(5):1031-40. doi: 10.1111/j.1440-1827.1984.tb07633.x.
Surgical specimens of the salivary gland tumor were studied by immunohistochemical techniques using the anti-keratin antibody and the anti-myosin antibody. In the normal tissue, keratin was localized predominantly in the duct epithelial cells and myosin in the myoepithelial cells. According to the immunohistochemical staining patterns, the tumors were able to be divided into two groups: one group consisted of pleomorphic adenoma, adenoid cystic carcinoma, and adenocarcinoma which showed a mixture of keratin- and myosin-positive cells, respectively, mimicking the structures of the intercalated duct; the other comprised monomorphic adenoma and mucoepidermoid tumor which disclosed keratin-positive cells predominantly, resembling the constituent of the excretory duct. These results were mostly consistent with the "bicellular theory" that the salivary tumors generate from the intercalated duct reserve cells and the excretory duct reserve cells.
利用抗角蛋白抗体和抗肌球蛋白抗体，通过免疫组织化学技术对唾液腺肿瘤的手术标本进行了研究。在正常组织中，角蛋白主要定位于导管上皮细胞，肌球蛋白定位于肌上皮细胞。根据免疫组织化学染色模式，肿瘤可分为两组：一组由多形性腺瘤、腺样囊性癌和腺癌组成，分别显示角蛋白阳性细胞和肌球蛋白阳性细胞的混合，模仿闰管的结构；另一组包括单形性腺瘤和黏液表皮样瘤，主要显示角蛋白阳性细胞，类似于排泄管的成分。这些结果大多与“双细胞理论”一致，即唾液腺肿瘤由闰管储备细胞和排泄管储备细胞产生。
