Chrapkowska K J
Acta Microbiol Pol. 1984;33(2):137-45.
Culture fluids obtained from submerged cultures of white, brown and gray wood rot fungi were assayed for the presence of cellulolytic activity complexes against the model substrated carboxymethylcellulose-Na and Standard Whatman cellulose and natural substrates, i.e. celluloses isolated from pine bark and sawdust. The cellulolytic activity of the examined fungal species was highly differentiated. The use of model and natural substrates allowed determination of the high substrate specificity of the cellulase complexes produced by the fungi. Not all the fungi were found to produce EC 3.2.1.4. endo-1, 4-beta-glucanase under the culture conditions employed. All the fungi were, however, able to produce a complex of EC 3.2.1.4. exo-1, 4-beta-glucanases. All the examined fungi were also able to degrade, although to a varied extent, such higher forms of cellulose as Standard Whatman cellulose or natural celluloses isolated from pine bark and sawdust. Determination of the cellulolytic activity of fungi against the above-mentioned specific natural substrates affords the possibility of their practical use.
对从白腐菌、褐腐菌和灰腐菌的深层培养物中获得的培养液进行分析，检测其对模型底物羧甲基纤维素钠、标准Whatman纤维素以及天然底物（即从松树皮和锯末中分离出的纤维素）的纤维素分解活性复合物。所检测的真菌种类的纤维素分解活性差异很大。使用模型底物和天然底物能够确定真菌产生的纤维素酶复合物具有很高的底物特异性。在所采用的培养条件下，并非所有真菌都能产生EC 3.2.1.4内切-1,4-β-葡聚糖酶。然而，所有真菌都能够产生EC 3.2.1.4外切-1,4-β-葡聚糖酶复合物。所有检测的真菌也都能够降解标准Whatman纤维素或从松树皮和锯末中分离出的天然纤维素等较高形式的纤维素，尽管降解程度各不相同。测定真菌对上述特定天然底物的纤维素分解活性为其实际应用提供了可能性。
