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粗糙脉孢菌qa基因簇中两个结构基因的5'非翻译序列。

5'-Untranslated sequences of two structural genes in the qa gene cluster of Neurospora crassa.

作者信息

Alton N K, Buxton F, Patel V, Giles N H, Vapnek D

出版信息

Proc Natl Acad Sci U S A. 1982 Mar;79(6):1955-9. doi: 10.1073/pnas.79.6.1955.

DOI:10.1073/pnas.79.6.1955
PMID:6210913
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC346100/
Abstract

The coding regions of two genes (qa-2 and qa-3) in the qa gene cluster of Neurospora crassa have been localized by nucleotide sequence analysis combined with data on previously determined NH2-terminal amino acid sequences for the proteins that these genes encode. The start point of transcription for each of these genes has been determined by nuclease S1 mapping experiments with poly(A)+RNA isolated from quinic acid-induced cultures of N. crassa. The sequences of approximately 200 nucleotides 5' to the start point of transcription have been compared with each other and with those of other eukaryotes. The results show that neither of these regions for the qa-2 nor the qa-3 genes share any significant homology with sequences apparently conserved in higher eukaryotic promoters (-25 and -70 regions). However, the qa-2 and qa-3 sequences do show homology with each other in these regions. Comparison of the 5'-flanking regions of these Neurospora genes with those of several Saccharomyces cerevisiae genes reveals a number of similarities in the region preceding the translation initiation codons.

摘要

通过核苷酸序列分析，并结合先前确定的这些基因所编码蛋白质的氨基末端氨基酸序列数据，已确定了粗糙脉孢菌qa基因簇中两个基因（qa - 2和qa - 3）的编码区。通过对从奎尼酸诱导的粗糙脉孢菌培养物中分离出的聚腺苷酸加尾RNA（poly(A)+RNA）进行核酸酶S1图谱分析实验，确定了这些基因各自的转录起始点。已将转录起始点上游约200个核苷酸的序列相互比较，并与其他真核生物的序列进行了比较。结果表明，qa - 2基因和qa - 3基因的这些区域与高等真核生物启动子中明显保守的序列（-25和-70区域）均无任何显著同源性。然而，qa - 2和qa - 3序列在这些区域彼此显示出同源性。将这些粗糙脉孢菌基因的5'侧翼区域与几种酿酒酵母基因的5'侧翼区域进行比较，发现在翻译起始密码子之前的区域存在一些相似之处。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e54/346100/3dd3472f2c11/pnas00445-0284-a.jpg

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