Montgomery D L, Leung D W, Smith M, Shalit P, Faye G, Hall B D
Proc Natl Acad Sci U S A. 1980 Jan;77(1):541-5. doi: 10.1073/pnas.77.1.541.
The two apocytochrome c proteins of yeast are coded for by separate genes. Iso-2-cytochrome c differs from the iso-1 protein at 17 positions within a homologous sequence of 108 amino acids. The previously cloned iso-1-cytochrome c coding sequence has been used to identify lambda-yeast recombinant phage containing the gene for iso-2-cytochrome c. The latter protein contains the dipeptide Ala-Ala which is coded for by the nucleic acid sequence G-C-N-G-C-N. The recognition specificity of restriction endonuclease Fnu4HI for G-C-N-G-C provided a rapid means of locating the region of the cloned fragment which codes for iso-2-cytochrome c. The DNA sequence of this gene has been determined and compared with that of the iso-1-cytochrome c locus. There is no intervening sequence within the gene for iso-2-cytochrome c. At 45 of the 91 positions for which iso-1- and iso-2-cytochrome c have the same amino acid, the codons differ. Such third position variation does not occur within the region coding for amino acids 70-80, the protein sequence that is also most conserved among all eukaryotic cytochromes c.
酵母的两种脱辅基细胞色素c蛋白由不同的基因编码。同工-2-细胞色素c与同工-1蛋白在108个氨基酸的同源序列中的17个位置上存在差异。先前克隆的同工-1-细胞色素c编码序列已被用于鉴定含有同工-2-细胞色素c基因的λ-酵母重组噬菌体。后一种蛋白含有由核酸序列G-C-N-G-C-N编码的二肽Ala-Ala。限制性内切酶Fnu4HI对G-C-N-G-C的识别特异性提供了一种快速定位克隆片段中编码同工-2-细胞色素c区域的方法。已确定该基因的DNA序列并将其与同工-1-细胞色素c基因座的序列进行了比较。同工-2-细胞色素c基因内没有间隔序列。在同工-1-和同工-2-细胞色素c具有相同氨基酸的91个位置中的45个位置上，密码子不同。这种第三位的变异在编码氨基酸70-80的区域内不发生，该蛋白质序列在所有真核细胞色素c中也是最保守的。
Zotero 插件