Melamed I, Zakuth V, Tzechoval E, Spirer Z
J Clin Lab Immunol. 1982 Apr;7(3):173-7.
The effect of splenectomy on the expression of suppressor activity of peripheral blood lymphocytes was examined in this study. 63 soldiers splenectomized for trauma or hematological diseases one to 24 years earlier, were studied by measuring the suppressor cell activity (using the Con A method), and lymphocyte blast transformation in response to lectines (PHA, Con A, PWM). There was a decrease in suppressor cell activity in 15 (23.8%) of the splenectomized patients. This decrease was greater in subjects splenectomized for trauma than in those splenectomized electively (24% and 22% respectively). The decrease was related to length to time from surgery being highest in the first 5 years after removal of the spleen and was more frequent in females (43%). The mean of the stimulation indices calculated from the mitogenic response to PHA, PWM and Con A was lower in the splenectomized patients with reduced suppressor activity than in those with normal suppressor activity. The findings of our present study supports the view that splenectomy interferes with normal immunoregulatory mechanisms and suggests that pyogenic infections are not the sole hazard of this procedure.
本研究检测了脾切除术对外周血淋巴细胞抑制活性表达的影响。对63名因创伤或血液系统疾病在1至24年前接受脾切除术的士兵进行了研究，通过测量抑制细胞活性（采用刀豆蛋白A法）以及淋巴细胞对凝集素（PHA、刀豆蛋白A、PWM）的增殖转化反应来进行研究。15名（23.8%）脾切除患者的抑制细胞活性降低。因创伤行脾切除术的患者其抑制细胞活性降低幅度大于择期脾切除患者（分别为24%和22%）。这种降低与手术时间长短有关，在脾切除后的头5年里最高，且在女性中更常见（43%）。与抑制活性正常的脾切除患者相比，抑制活性降低的脾切除患者中，由对PHA、PWM和刀豆蛋白A的促有丝分裂反应计算出的刺激指数平均值更低。我们目前的研究结果支持这样一种观点，即脾切除术会干扰正常的免疫调节机制，并表明化脓性感染并非该手术的唯一危害。
