Tomino S, Fujiwara H, Kagimoto T, Mitsuya H, Nishimura H, Kishimoto S
Clin Exp Immunol. 1982 Jun;48(3):625-32.
Hypergammaglobulinaemia (HGG) is frequently found in patients with hepatic cirrhosis (HC). Using an assay system of in vitro PWM-stimulated immunoglobulin (Ig) production, the amounts of IgG, IgA, and IgM produced by peripheral blood lymphocytes (PBL) from 15 HBs Ag-negative patients with HC and from 16 age-matched healthy subjects were quantitated by radioimmunoassay. We found that PBL from patients with HC produced significantly greater amounts of IgG (P less than 0.05) but not IgA or IgM than did those from control subjects. This increased IgG production by PBL from patients with HC was attributed to enhanced T helper activity and not to enhanced B cell function. We also searched for defects in naturally occurring suppressor T cell activity which is sensitive to irradiation. Irradiation-induced enhancement for IgG production was significantly lower in patients with HC compared with age-matched control subjects (P less than 0.01). Similarly, we examined the effect of Con A-induced suppressor T cells on the in vitro PWM-stimulated IgG production by allogeneic PBL and observed the decrease of Con A-induced suppressor T cell activity in patients with HC (P = 0.01). We conclude, therefore, that the increased serum levels of Ig, particularly IgG in patients with HC may result from in part on the basis of depressed ability of naturally occurring suppressor T cells or Con A-induced suppressor T cells to suppress Ig production.
高球蛋白血症（HGG）在肝硬化（HC）患者中很常见。使用体外PWM刺激的免疫球蛋白（Ig）产生检测系统，通过放射免疫测定法定量了15例HBs Ag阴性的HC患者和16例年龄匹配的健康受试者外周血淋巴细胞（PBL）产生的IgG、IgA和IgM量。我们发现，HC患者的PBL产生的IgG量显著高于对照组（P<0.05），但IgA或IgM量则不然。HC患者PBL产生的IgG增加归因于T辅助活性增强，而非B细胞功能增强。我们还研究了对辐射敏感的自然存在的抑制性T细胞活性的缺陷。与年龄匹配的对照组相比，HC患者辐射诱导的IgG产生增强明显较低（P<0.01）。同样，我们检测了Con A诱导的抑制性T细胞对同种异体PBL体外PWM刺激的IgG产生的影响，观察到HC患者Con A诱导的抑制性T细胞活性降低（P = 0.01）。因此，我们得出结论，HC患者血清Ig水平升高，尤其是IgG升高，可能部分是由于自然存在的抑制性T细胞或Con A诱导的抑制性T细胞抑制Ig产生的能力下降所致。
