Parker D, Turk J L
Immunology. 1982 Sep;47(1):61-6.
Cyclophosphamide (300 mg/kg) given before or up to 2 days after sensitization, induces increased contact skin reactions at 8 days. Reactions were suppressed with cyclophosphamide (CY) given between 3 and 5 days after sensitization; reactivity returned on day 10. CY, given on days 6 to 8, only suppressed reactions when skin tests were made 4 days later. This temporary depression of contact sensitivity corresponds with the maximal reduction of peripheral blood lymphocytes. CY given 1-2 days after DNFB produced decreased T-cell proliferation in local lymph nodes 4 days after sensitization. CY given 3 days after DNFB produced maximal T-cell suppression on 5 day nodes. Massive increase in T-cell proliferation in 5 day nodes occurred when CY was given on the day of sensitization or the day before. Thus CY given around sensitization acts mainly on suppressor cells whereas given later, the action is principally on the effector functions.
致敏前或致敏后2天内给予环磷酰胺(300mg/kg),可使8天时的接触性皮肤反应增强。致敏后3至5天给予环磷酰胺(CY)可抑制反应;10天时反应性恢复。致敏后6至8天给予CY,仅在4天后进行皮肤试验时抑制反应。这种接触敏感性的暂时降低与外周血淋巴细胞的最大减少相对应。DNFB后1 - 2天给予CY可使致敏后4天局部淋巴结中的T细胞增殖减少。DNFB后3天给予CY可使5天的淋巴结中T细胞受到最大抑制。致敏当天或前一天给予CY时,5天的淋巴结中T细胞增殖大量增加。因此,致敏前后给予CY主要作用于抑制细胞,而稍后给予则主要作用于效应功能。