Nagasawa T, Abe T, Hanada T
Scand J Haematol. 1982 May;28(5):389-98. doi: 10.1111/j.1600-0609.1982.tb00544.x.
Coculture with mononuclear cells from aplastic anaemia (AA) patients and normal bone marrow cells produced different responses among 10 patients studied. In 4 cases, CFU-E growth was inhibited while in another 2 cases CFU-C growth was inhibited. To determine the suppressive effects of mononuclear cells from 6 AA patients, mononuclear cells were separated into T cells, B cells, and monocytes. It was found that T cells from 6 AA patients exhibited suppressive effects on normal CFU-C or CFU-E growth, whereas T cells from normal subjects enhanced the colony formation of CFU-C and CFU-E. On the other hand, monocytes fom 6 AA patients and normal subjects strongly suppressed normal CFU-C and CFU-E growth, although no significant difference was observed in both groups. The immune system in 10 AA patients was evaluated, 3 AA patients (Cases No. 1, 6, 8) exhibited an increase in Tr cells, impairment of PHA responsiveness, and low level of immunoglobulin. T cells from these patients showed a significant suppressive effect on PWM-induced differentiation of allogenic normal B cells. Thus, T cells from 3 out of 10 AA patients exhibited suppressor activities on both haematopoietic cell differentiation and B cell differentiation into plasma cells in vitro.
与再生障碍性贫血（AA）患者的单核细胞和正常骨髓细胞共培养，在研究的10例患者中产生了不同的反应。4例中CFU-E生长受到抑制，另外2例中CFU-C生长受到抑制。为了确定6例AA患者单核细胞的抑制作用，将单核细胞分离为T细胞、B细胞和单核细胞。发现6例AA患者的T细胞对正常CFU-C或CFU-E生长表现出抑制作用，而正常受试者的T细胞增强了CFU-C和CFU-E的集落形成。另一方面，6例AA患者和正常受试者的单核细胞均强烈抑制正常CFU-C和CFU-E生长，尽管两组之间未观察到显著差异。对10例AA患者的免疫系统进行了评估，3例AA患者（病例编号1、6、8）表现出Tr细胞增加、PHA反应性受损和免疫球蛋白水平降低。这些患者的T细胞对PWM诱导的同种异体正常B细胞分化表现出显著的抑制作用。因此，10例AA患者中有3例的T细胞在体外对造血细胞分化和B细胞分化为浆细胞均表现出抑制活性。
