线粒体外膜的结构：粗糙脉孢菌线粒体的外膜组分中孔状亚基的有序排列。
Structure of the outer mitochondrial membrane: ordered arrays of porelike subunits in outer-membrane fractions from Neurospora crassa mitochondria.
作者信息
Mannella C A
出版信息
J Cell Biol. 1982 Sep;94(3):680-7. doi: 10.1083/jcb.94.3.680.
Abstract
Light-membrane fractions obtained by hypoosmotic lysis of neurospora crassa mitochondria exhibit buoyant densities and marker-enzyme activities characteristic of outer mitochondrial membranes. SDS PAGE of these membrane fractions indicates that a polypeptide of M(r) 31,000 is the main protein component. Under negative-stain electron microscope examination many of the membranes in these fractions appear as large (0.5-1- mum diameter), collapsed vesicles. The surfaces of flattened, open (i.e., ripped) vesicles often exhibit extended two-dimensional arrays of subunits are arranged into hexagons within each parallelogram unit cell, 12.6x11.1 nm (lattice angle = 109 degrees).
摘要
通过低渗裂解粗糙脉孢菌线粒体获得的轻膜组分呈现出具有线粒体外膜特征的浮力密度和标志酶活性。这些膜组分的SDS-PAGE表明，分子量为31,000的一种多肽是主要的蛋白质成分。在负染电子显微镜检查下，这些组分中的许多膜呈现为大的（直径0.5 - 1μm）、塌陷的囊泡。扁平的、开放的（即撕开的）囊泡表面常常呈现出亚基的二维延伸阵列，这些亚基在每个平行四边形晶胞（12.6×11.1 nm，晶格角 = 109度）内排列成六边形。
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