Dienstag J L, Savarese A M, Cohen R B, Bhan A K
Clin Exp Immunol. 1982 Oct;50(1):7-16.
To evaluate the interactions between circulating immune complexes (CIC) and lymphoid cells in primary biliary cirrhosis (PBC), we determined (1) whether antibodies to lymphocytes in PBC serum, independent of CIC, could account for binding in the Raji cell assay for CIC and (2) whether CIC or other humoral factors in PBC serum could interact with lymphoid cells to alter their function. We found that three quarters of CIC positive PBC sera bound specifically to Raji cells via complement receptors, while only one quarter had antibodies to lymphoid cells or Raji cells devoid of complement receptors. We also demonstrated factors which inhibited cell-mediated cytotoxicity and suppressor cell activity in PBC sera; however, we found no correlation between the level and presence of CIC or of lymphocyte antibodies and the level or presence of these serum inhibitory factors. Thus, although the detection of CIC in PBC is not artifactual, the contribution of CIC and other serum factors to the other immunological aberrations in PBC remains to be elucidated.
为评估原发性胆汁性肝硬化（PBC）中循环免疫复合物（CIC）与淋巴细胞之间的相互作用，我们确定了：（1）PBC血清中针对淋巴细胞的抗体，不依赖于CIC，是否能解释在Raji细胞检测CIC时的结合情况；（2）PBC血清中的CIC或其他体液因子是否能与淋巴细胞相互作用以改变其功能。我们发现，四分之三CIC阳性的PBC血清通过补体受体特异性结合Raji细胞，而只有四分之一的血清含有针对淋巴细胞或无补体受体的Raji细胞的抗体。我们还证实了PBC血清中存在抑制细胞介导的细胞毒性和抑制细胞活性的因子；然而，我们发现CIC或淋巴细胞抗体的水平及存在与否与这些血清抑制因子的水平或存在与否之间并无相关性。因此，虽然在PBC中检测到CIC并非人为因素所致，但CIC和其他血清因子对PBC中其他免疫异常的作用仍有待阐明。
