Tutas D J, Paoletti E
J Virol. 1978 Jan;25(1):37-41. doi: 10.1128/JVI.25.1.37-41.1978.
Synthesis of polynucleotide 5'-triphosphatase, which is presumably involved in the initial modification in the series of reactions by which 5'-termini of vaccinia mRNA become capped and methylated, has been demonstrated in vaccinia virus infected HeLa cells. Synthesis of the enzyme is prevented by actinomycin D and cycloheximide, suggesting that both de novo DNA-dependent RNA and protein syntheses are required. On the other hand, cytosine arabinoside, an inhibitor of viral DNA replication, does not prevent induction of the enzyme. The latter observation, together with the kinetics of synthesis of the enzyme in vaccinia virus-infected HeLa cells, suggests that polynucleotide 5'-triphosphatase is an "early" or prereplicative viral protein. Immunologlobulin produced against the purified virion-associated polynucleotide 5'-triphosphatase as antigen neutralized the activity of the induced polynucleotide 5'-triphosphatase, thus indicating the identity of the two enzymes.
在感染痘苗病毒的HeLa细胞中已证实了多核苷酸5'-三磷酸酶的合成，该酶可能参与了痘苗病毒mRNA 5'-末端加帽和甲基化系列反应的初始修饰过程。放线菌素D和环己酰亚胺可抑制该酶的合成，这表明从头开始的依赖DNA的RNA合成和蛋白质合成均是必需的。另一方面，病毒DNA复制抑制剂阿糖胞苷并不能阻止该酶的诱导合成。后一观察结果，连同该酶在痘苗病毒感染的HeLa细胞中的合成动力学，提示多核苷酸5'-三磷酸酶是一种“早期”或复制前病毒蛋白。以纯化的病毒体相关多核苷酸5'-三磷酸酶作为抗原产生的免疫球蛋白中和了诱导的多核苷酸5'-三磷酸酶的活性，从而表明这两种酶是相同的。
