Moalic J M, Aumont M C, Bercovici J, Swynghedauw B
Reprod Nutr Dev (1980). 1981;21(2):289-92.
The fractional turnover rate of the total protein extracted from cardiac rat ventricles has been measured by the continuous infusion flow technique in unanesthesized animals using 3H-lysine as a marker. This turnover rate approximated 12 p. 100 per day, doubled after aortic stenosis or aortic incompetence, and reached a peak within a week after stenosis and 2 weeks after incompetence. The fractional turnover rate of actin and myosin heavy-chains has been measured by the same technique using 14C-tyrosine as a marker and preparative gel electrophoresis to isolate the protein. This turnover rate approximated 22 and 9 p. 100 per day for myosin and actin, respectively, and doubled 4 days after aortic stenosis. It is suggested that a drop in contraction efficiency triggered protein synthesis in cardiac overloading.
采用连续输注流动技术，以3H-赖氨酸为标记物，在未麻醉的动物中测量了从大鼠心脏心室提取的总蛋白的分数周转率。该周转率约为每天12%，在主动脉狭窄或主动脉瓣关闭不全后翻倍，并在狭窄后一周内和关闭不全后两周内达到峰值。采用相同技术，以14C-酪氨酸为标记物，并通过制备性凝胶电泳分离蛋白质，测量了肌动蛋白和肌球蛋白重链的分数周转率。肌球蛋白和肌动蛋白的该周转率分别约为每天22%和9%，在主动脉狭窄后4天翻倍。有人提出，收缩效率的下降触发了心脏超负荷时的蛋白质合成。
