Moalic J M, Bercovici J, Swynghedauw B
Cardiovasc Res. 1981 Sep;15(9):515-21. doi: 10.1093/cvr/15.9.515.
The rate of protein synthesis in the heart of normal rats and those with either abdominal aortic stenosis (AS), aortic incompetence (AI) or both AS + AI, was measured by a continuous infusion of (3H) lysine. The total protein synthesis rate in normal animals averaged 13% per day. During bot types of hypertrophy, there was an increased incorporation of (3H) lysine into proteins without any significant change in the specific radioactivity of free lysine in the tissue. After 6 days of stenosis, the synthesis rate of total mixed RNA-free proteins of the two ventricles had increased to 24.8% per day, and returned to normal by the second week. After aortic incompetence, the average fractional rate of protein synthesis was near normal during the first week and a significant increase (up to 28% per day) was observed only after 2 to 3 weeks. Protein synthesis returned to normal by the first month. The rate of protein synthesis was normal in the final stage of cardiac overloading comparable to that obtained when both stenosis and incompetence had been combined. These results were in agreement with in vitro studies which showed a normal protein synthesis rate during the first hours of volume overloading It was hypothesised that the trigger for protein synthesis in both conditions was a decline in efficiency due to a change in the speed of shortening.
通过持续输注（3H）赖氨酸来测定正常大鼠以及患有腹主动脉狭窄（AS）、主动脉瓣关闭不全（AI）或两者兼具（AS + AI）的大鼠心脏中的蛋白质合成速率。正常动物的总蛋白质合成速率平均为每天13%。在两种类型的肥大过程中，（3H）赖氨酸掺入蛋白质的量增加，而组织中游离赖氨酸的比放射性没有任何显著变化。狭窄6天后，两个心室的总无混合RNA蛋白质的合成速率增加到每天24.8%，并在第二周恢复正常。主动脉瓣关闭不全后，蛋白质合成的平均分数速率在第一周接近正常，仅在2至3周后才观察到显著增加（高达每天28%）。蛋白质合成在第一个月恢复正常。在心脏超负荷的最后阶段，蛋白质合成速率正常，这与狭窄和关闭不全同时存在时的情况相当。这些结果与体外研究一致，体外研究表明容量超负荷的最初几个小时内蛋白质合成速率正常。据推测，在这两种情况下蛋白质合成的触发因素是由于缩短速度的变化导致效率下降。
