Craggs R I, Peel S
J Reprod Immunol. 1983 Jan;5(1):27-37. doi: 10.1016/0165-0378(83)90018-9.
Cells from metrial glands of pregnant rats were examined for surface receptors. No E, EA mu or EAC receptors were demonstrated. Fc gamma receptors were detected by EA gamma rosette formation, using sheep red blood cells sensitised with the IgG fraction of rabbit or rat antisera. Significantly more of the metrial gland cells, and of the rat peritoneal exudate and spleen cells examined as controls, formed rosettes with red cells sensitised with rabbit IgG than with those sensitised with rat IgG. The proportion of metrial gland cells forming EA gamma rosettes decreased significantly between day 12 and day 15 of pregnancy but increased by day 19. Metrial gland cells from deciduomata formed EA gamma rosettes, and the proportions varied during pseudopregnancy. At day 13 of pregnancy a greater proportion of metrial gland cells displayed Fc gamma receptors in multiparous rats than in primigravid rats. The binding affinity of the Fc gamma receptors was characterised by inhibition studies with homologous and heterologous IgGs. Maximal inhibition occurred when the inhibitory IgG was homologous to the IgG used to sensitise the red cells. EA gamma rosette formation by cells from the metrial gland was inhibited by both monomeric and heat-aggregated IgGs.
对怀孕大鼠子宫内膜腺细胞进行表面受体检测。未发现雌激素（E）、μ 型雌激素结合蛋白（EA mu）或补体结合性雌激素（EAC）受体。通过用兔或大鼠抗血清的 IgG 组分致敏的绵羊红细胞形成 EAγ 玫瑰花结来检测 Fcγ 受体。作为对照检测的子宫内膜腺细胞、大鼠腹腔渗出细胞和脾细胞中，与兔 IgG 致敏红细胞形成玫瑰花结的细胞明显多于与大鼠 IgG 致敏红细胞形成玫瑰花结的细胞。在怀孕第 12 天至第 15 天期间，形成 EAγ 玫瑰花结的子宫内膜腺细胞比例显著下降，但到第 19 天有所增加。来自蜕膜瘤的子宫内膜腺细胞形成 EAγ 玫瑰花结，且在假孕期间比例有所变化。在怀孕第 13 天，经产大鼠中显示 Fcγ 受体的子宫内膜腺细胞比例高于初孕大鼠。通过用同源和异源 IgG 进行抑制研究来表征 Fcγ 受体的结合亲和力。当抑制性 IgG 与用于致敏红细胞的 IgG 同源时，发生最大抑制。子宫内膜腺细胞形成 EAγ 玫瑰花结的过程受到单体 IgG 和热聚集 IgG 的抑制。
