Daki N M, Stewart I J, Wild A E
Department of Biology, Faculty of Science, University of Southampton, U.K.
J Reprod Immunol. 1989 Dec;16(3):249-60. doi: 10.1016/0165-0378(89)90054-5.
Single cells prepared from metrial glands of mice killed at days 10, 13 and 17 of pregnancy were assayed for the expression of Fc gamma receptors in a standard rosetting assay using sheep red blood cells sensitised with a mouse monoclonal IgG2b antibody. Rosettes, indicating Fc gamma receptors, were found on both granulated metrial gland (GMG) cells and non-GMG cells, comprising mainly stromal cells, from each stage of pregnancy. Some animals were given an intravenous injection of horseradish peroxidase 2 h before they were killed in order to identify endocytic cells. No GMG cells were found to have endocytosed the horseradish peroxidase. Non-GMG cells which showed endocytic activity all expressed Fc gamma receptors but these receptors were also found on some of the non-GMG cells which had not exhibited endocytosis. The finding of Fc gamma receptors on GMG cells provides further evidence that these cells may be related to NK cells.
从小鼠妊娠第10、13和17天处死时的子宫蜕膜制备的单细胞，采用用小鼠单克隆IgG2b抗体致敏的绵羊红细胞，通过标准玫瑰花结试验检测Fcγ受体的表达。在妊娠各阶段的颗粒状子宫蜕膜（GMG）细胞和主要由基质细胞组成的非GMG细胞上均发现了指示Fcγ受体的玫瑰花结。为了鉴定内吞细胞，一些动物在处死前2小时静脉注射辣根过氧化物酶。未发现GMG细胞内吞辣根过氧化物酶。表现出内吞活性的非GMG细胞均表达Fcγ受体，但在一些未表现出内吞作用的非GMG细胞上也发现了这些受体。GMG细胞上存在Fcγ受体这一发现进一步证明这些细胞可能与NK细胞有关。
