Kavanagh J P, Darby C
J Reprod Fertil. 1983 May;68(1):51-6. doi: 10.1530/jrf.0.0680051.
A creatine kinase assay based on estimation of creatine liberated from creatine phosphate was accurate and reproducible for use with seminal or prostatic fluid, after allowance was made for acid phosphatase interference. Comparison of this method with one which relies on enzymic coupling of ATP formation to NADP+ oxidation shows that the latter under-estimates creatine kinase activity by a factor of about 3. This discrepancy could be due to the high ATPase activity found in prostatic and seminal fluid. Uncritical use of the NADP+ assay might account for different seminal creatine kinase values reported in the literature. Interrelationships between ATPase, creatine kinase and zinc suggest that seminal ATPase is a prostatic secretory product while creatine kinase may be multiglandular in origin.
一种基于对从磷酸肌酸中释放出的肌酸进行估算的肌酸激酶测定法，在考虑了酸性磷酸酶干扰后，用于精液或前列腺液时准确且可重复。将该方法与一种依赖于ATP形成与NADP⁺氧化的酶促偶联的方法进行比较表明，后者将肌酸激酶活性低估了约3倍。这种差异可能是由于在前列腺液和精液中发现的高ATP酶活性。不加区分地使用NADP⁺测定法可能是文献中报道的精液肌酸激酶值不同的原因。ATP酶、肌酸激酶和锌之间的相互关系表明，精液ATP酶是前列腺的分泌产物，而肌酸激酶可能起源于多个腺体。
