人精浆中岩藻糖基转移酶的前列腺来源——对健康对照者以及不育或前列腺癌男性的研究

Prostatic origin of fucosyl transferase in human seminal plasma--a study on healthy controls and on men with infertility or with prostatic cancer.

作者信息

Ronquist G, Stegmayr B

出版信息

Urol Res. 1984;12(5):243-7. doi: 10.1007/BF00256148.

Abstract

Fucosyl transferase was recovered in soluble form in human seminal plasma. The enzyme had very little activity, as it was structurally bound to prostasomes, which are membrane-surrounded organelles in seminal plasma. The fucosyl transferase activity was recorded on Sephadex G200 chromatography of seminal plasma (supernatant after ultracentrifugation) in one single peak coinciding with that of prostate-specific acid phosphatase. Studies on healthy men and on men with prostatic cancer suggest a prostatic origin of fucosyl transferase activity; two of the men with prostatic cancer displayed 50-95% decreased activities. Antiandrogenic therapy in another man with cancer resulted in substantial reductions in seminal plasma contents of fucosyl transferase, ATPase, acid phosphatase and fructose suggesting a role of testosterone in their secretions.

摘要

岩藻糖基转移酶以可溶形式存在于人类精浆中。该酶活性很低，因为它在结构上与前列腺小体结合，前列腺小体是精浆中被膜包围的细胞器。在对精浆（超速离心后的上清液）进行葡聚糖凝胶G200层析时，岩藻糖基转移酶活性记录在与前列腺特异性酸性磷酸酶相同的单一峰中。对健康男性和前列腺癌男性的研究表明，岩藻糖基转移酶活性起源于前列腺；两名前列腺癌男性的酶活性降低了50 - 95%。另一名癌症男性接受抗雄激素治疗后，精浆中岩藻糖基转移酶、ATP酶、酸性磷酸酶和果糖的含量大幅降低，这表明睾酮在它们的分泌过程中起作用。