Meroni P L, Balestrieri G, Barcellini W, DeBartolo G, Fenini G, Tincani A, Zanussi C
J Clin Lab Immunol. 1983 Mar;10(3):159-63.
Con A induced suppressor cell assay was further characterized in this study. Significant lower mitogenic responses of autologous fresh cells cocultured with Con A-activated cells were found when compared with the response of cocultures of responders plus control cells preincubated in medium alone. However, Con A-activated cells did not express a real suppression, since no difference was found between responses of fresh responders alone and responses of cocultures with Con A-activated cells. So, Con A activation seemed to block the expression of the enhancement provided by control cells to autologous responders, rather than to induce a real suppressor activity. The induction of the Con A suppressor cell activity required cell proliferation but it was not proportional to the degree of DNA synthesis. Monocyte depleted cell populations exhibited lower Con A suppressor cell activity compared to unfractionated cells, suggesting a cooperative role of monocytes in the Con A induction step. The expression of this activity was not due to a cytotoxicity against the responders and was dependent on the number of activated cells added. Mononuclear cells from active lupus erythematosus (SLE) patients, but not from inactive SLE patients differed from normal controls in showing a significant loss of suppression index.
本研究进一步对刀豆蛋白A诱导的抑制细胞试验进行了特性分析。与仅在培养基中预孵育的反应细胞与对照细胞共培养的反应相比，发现与刀豆蛋白A激活细胞共培养的自体新鲜细胞的促有丝分裂反应显著降低。然而，刀豆蛋白A激活的细胞并未表现出真正的抑制作用，因为单独新鲜反应细胞的反应与与刀豆蛋白A激活细胞共培养的反应之间未发现差异。因此，刀豆蛋白A激活似乎阻断了对照细胞对自体反应细胞提供的增强作用的表达，而不是诱导真正的抑制活性。刀豆蛋白A抑制细胞活性的诱导需要细胞增殖，但与DNA合成程度不成比例。与未分离的细胞相比，去除单核细胞的细胞群体表现出较低的刀豆蛋白A抑制细胞活性，这表明单核细胞在刀豆蛋白A诱导步骤中具有协同作用。这种活性的表达不是由于对反应细胞的细胞毒性，并且依赖于添加的激活细胞的数量。活动期系统性红斑狼疮（SLE）患者的单核细胞与非活动期SLE患者及正常对照不同，表现出显著的抑制指数降低。
