Fernandez L A, Macsween J M
J Immunol. 1980 Jul;125(1):267-9.
Significantly lower mitogenic responses of fresh cells co-cultured with Con A-stimulated cells were found when compared with the responses of fresh cells co-cultured with preincubated control cells. We do not agree with the interpretation that this effect represents the generation of suppressor cells by Con A, since the responses of fresh cells cultured alone were also significantly less than when co-cultured with control cells and the same as when co-cultured with the Con A-stimulated cells. Treatment with mitomycin C was sufficient to prevent the preincubated cells from contributing to the mitogenic response of the fresh cells. The increased responses of fresh cells when co-cultured with preincubated cells seems analagous to the increased mitogenic responses of cells aged in vitro by preincubation without mitogen. This effect seems to be transferable to fresh cells in the absence of cell division. Although preincubation in the presence of Con A abrogates this effect, we do not interpret this as the generation of suppressor cells.
与与预孵育对照细胞共培养的新鲜细胞的反应相比，发现与伴刀豆球蛋白A（Con A）刺激的细胞共培养的新鲜细胞的促有丝分裂反应显著降低。我们不同意这种效应代表Con A产生抑制细胞的解释，因为单独培养的新鲜细胞的反应也明显低于与对照细胞共培养时，且与与Con A刺激的细胞共培养时相同。用丝裂霉素C处理足以阻止预孵育细胞对新鲜细胞的促有丝分裂反应产生影响。新鲜细胞与预孵育细胞共培养时反应的增加似乎类似于通过无丝裂原预孵育在体外老化的细胞促有丝分裂反应增加。这种效应似乎在没有细胞分裂的情况下可转移到新鲜细胞。尽管在Con A存在下预孵育可消除这种效应，但我们不将此解释为抑制细胞的产生。
