Manzella J P, Clark J K
Antimicrob Agents Chemother. 1983 Mar;23(3):360-3. doi: 10.1128/AAC.23.3.360.
The effect of moxalactam and cefuroxime on mitogen-stimulated human peripheral blood mononuclear leukocytes was studied. Mononuclear leukocytes, mitogen, and antibiotic were added to microtiter wells. Cells were cultured for 3 days, pulsed with tritiated thymidine, and then counted. Compared with control cell cultures, treated cultures showed phytohemagglutinin responsiveness to be depressed by the addition of moxalactam at concentrations of 25 to 200 micrograms/ml (P less than 0.001) and by cefuroxime at concentrations of 50 to 200 micrograms/ml (P less than 0.02 to P less than 0.01). The depressive effect on blastogenesis was less marked when concanavalin A was used. Unstimulated lymphocyte transformation responses were also depressed by both antibiotics at all concentrations (P less than 0.05). Preincubation of mononuclear leukocytes with antibiotic for 2 h, followed by washing and culturing in an antibiotic-free medium, did not depress transformation response. When antibiotic was added 24 h after mitogen, depression of response was insignificant. The data from this study suggest that two new beta-lactam antibiotics, at concentrations achievable in serum when used therapeutically, may have immunosuppressant effects. It remains to be established whether these effects are clinically important.
研究了羟羧氧酰胺菌素和头孢呋辛对丝裂原刺激的人外周血单个核白细胞的作用。将单个核白细胞、丝裂原和抗生素加入微量滴定板孔中。细胞培养3天，用氚标记的胸腺嘧啶核苷脉冲处理，然后进行计数。与对照细胞培养物相比，在25至200微克/毫升浓度下添加羟羧氧酰胺菌素（P<0.001）以及在50至200微克/毫升浓度下添加头孢呋辛（P<0.02至P<0.01）时，处理后的培养物显示植物血凝素反应性受到抑制。当使用刀豆球蛋白A时，对细胞分裂的抑制作用不太明显。在所有浓度下，两种抗生素对未刺激的淋巴细胞转化反应也有抑制作用（P<0.05）。将单个核白细胞与抗生素预孵育2小时，然后洗涤并在无抗生素的培养基中培养，不会抑制转化反应。当在丝裂原加入24小时后添加抗生素时，反应的抑制作用不明显。这项研究的数据表明，两种新型β-内酰胺类抗生素在治疗使用时血清中可达到的浓度下可能具有免疫抑制作用。这些作用在临床上是否重要还有待确定。
