Leyhausen G, Seibert G, Maidhof A, Müller W E
Antimicrob Agents Chemother. 1984 Nov;26(5):752-6. doi: 10.1128/AAC.26.5.752.
The in vitro effect of three cephalosporins (cefodizime, cefotaxime, and ceftizoxime) on the growth of the following lymphocytes or their derivatives was tested: L 5178y mouse lymphoma cells, Molt-4 cells, and murine splenic lymphocytes. Within the concentration range of 0.1 to 50 microM, the cephalosporins had no effect on L 5178y cell growth. However, Molt-4 cell growth was significantly stimulated by 0.3 to 20 microM cefotaxime and cefodizime but was not influenced by ceftizoxime. Binding studies with [14C]cefotaxime revealed that the Molt-4 cells responding to the drug bind this cephalosporin to their cell surface (1.9 X 10(5) molecules per cell); no significant binding was observed in the assays with L 5178y cells. Determinations of the extractable activities of DNA-synthesizing enzymes from cefodizime-treated Molt-4 cells showed a direct correlation between cell growth and DNA polymerase alpha as well as terminal deoxynucleotidyl transferase activity; the DNA polymerase beta activity remained unchanged. Cefodizime (0.15 to 50 microM) which was added to mouse spleen cell cultures significantly increased [3H]thymidine incorporation into lymphocytes. This stimulatory effect was less pronounced in concanavalin A-stimulated cultures. These findings suggest that some cephalosporins display a growth-stimulating influence on some lymphocyte populations.
测试了三种头孢菌素（头孢地嗪、头孢噻肟和头孢唑肟）对以下淋巴细胞或其衍生物生长的体外作用：L 5178y小鼠淋巴瘤细胞、Molt-4细胞和小鼠脾淋巴细胞。在0.1至50微摩尔的浓度范围内，头孢菌素对L 5178y细胞生长无影响。然而，0.3至20微摩尔的头孢噻肟和头孢地嗪显著刺激Molt-4细胞生长，但头孢唑肟对其无影响。用[14C]头孢噻肟进行的结合研究表明，对该药物有反应的Molt-4细胞将这种头孢菌素结合到其细胞表面（每个细胞1.9×10(5)个分子）；在对L 5178y细胞的检测中未观察到明显的结合。对头孢地嗪处理的Molt-4细胞中DNA合成酶的可提取活性的测定表明，细胞生长与DNA聚合酶α以及末端脱氧核苷酸转移酶活性之间存在直接相关性；DNA聚合酶β活性保持不变。添加到小鼠脾细胞培养物中的头孢地嗪（0.15至50微摩尔）显著增加了[3H]胸腺嘧啶核苷掺入淋巴细胞的量。在伴刀豆球蛋白A刺激的培养物中，这种刺激作用不太明显。这些发现表明，某些头孢菌素对某些淋巴细胞群体具有生长刺激作用。
