Herrmann F, Sieber G, Jauer B, Lochner A, Komischke B, Rühl H
Blut. 1983 Jul;47(1):41-51. doi: 10.1007/BF00321049.
The number of B lymphocytes, T lymphocytes and their helper/inducer, cytotoxic/suppressor and NK/K subpopulations was measured in peripheral blood and spleen cell suspensions from patients with Hodgkin's disease (HD) in the active stage of the disease and in remission status, as well as in Non-Hodgkin lymphomas (NHL) in active stage of the disease. B lymphocytes were determined by direct immunofluorescence and T lymphocytes with the E rosette technique. Helper/inducer, cytotoxic/suppressor, and NK/K T lymphocytes were determined by indirect immunofluorescence with the monoclonal antibodies OKT4, OKT8 and Leu 7 (HNK1). In the same way, Lyt3 was used for determination of the total T lymphocytes. Whereas in peripheral blood of the NHL group an increase of B lymphocytes and a slight reduction of T lymphocytes could be observed, with normal distribution of the subpopulations, in patients with active HD as well as in those in remission, a marked absolute and relative decrease of T helper/inducer cells was found with normal cytotoxic/suppressor and NK/K proportion. In contrast to this, a significant increase of helper/inducer T lymphocytes with decreased cytotoxic/suppressor T proportion was found in spleen cell suspensions of patients with HD.
对处于疾病活动期和缓解期的霍奇金病（HD）患者以及处于疾病活动期的非霍奇金淋巴瘤（NHL）患者的外周血和脾细胞悬液中的B淋巴细胞、T淋巴细胞及其辅助/诱导、细胞毒性/抑制和NK/K亚群的数量进行了测定。通过直接免疫荧光法测定B淋巴细胞，通过E花环技术测定T淋巴细胞。使用单克隆抗体OKT4、OKT8和Leu 7（HNK1）通过间接免疫荧光法测定辅助/诱导、细胞毒性/抑制和NK/K T淋巴细胞。同样，使用Lyt3测定总T淋巴细胞。在NHL组的外周血中，可以观察到B淋巴细胞增加，T淋巴细胞略有减少，亚群分布正常；而在活动期HD患者以及缓解期患者中，发现辅助/诱导T细胞绝对和相对明显减少，细胞毒性/抑制和NK/K比例正常。与此相反，在HD患者的脾细胞悬液中，发现辅助/诱导T淋巴细胞显著增加，细胞毒性/抑制T比例降低。
