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噬菌体λ蛋白cII与DNA螺旋上与RNA聚合酶相对的那一面的启动子结合。

Bacteriophage lambda protein cII binds promoters on the opposite face of the DNA helix from RNA polymerase.

作者信息

Ho Y S, Wulff D L, Rosenberg M

出版信息

Nature. 1983;304(5928):703-8. doi: 10.1038/304703a0.

DOI:10.1038/304703a0
PMID:6225025
Abstract

The bacteriophage lambda transcriptional activator protein, cII, coordinately regulates transcription from two phage promoters that control lysogenic development. We demonstrate that cII is a DNA binding protein that selectively interacts with a repeat sequence in the -35 region of the promoter. Furthermore, cII is shown to bind mainly one face of the DNA helix and to make its contacts primarily in the major groove of the DNA. RNA polymerase sees this same region from the opposite side and sandwiches the DNA helix between itself and cII.

摘要

噬菌体λ转录激活蛋白cII协同调节来自两个控制溶原性发育的噬菌体启动子的转录。我们证明cII是一种DNA结合蛋白，它选择性地与启动子-35区域的重复序列相互作用。此外，cII主要结合在DNA螺旋的一个面上，并主要在DNA的大沟中进行接触。RNA聚合酶从相反的一侧识别同一区域，并将DNA螺旋夹在自身与cII之间。

相似文献

1
Bacteriophage lambda protein cII binds promoters on the opposite face of the DNA helix from RNA polymerase.噬菌体λ蛋白cII与DNA螺旋上与RNA聚合酶相对的那一面的启动子结合。
Nature. 1983;304(5928):703-8. doi: 10.1038/304703a0.
2
Role of the RNA polymerase alpha subunits in CII-dependent activation of the bacteriophage lambda pE promoter: identification of important residues and positioning of the alpha C-terminal domains.RNA聚合酶α亚基在噬菌体λ pE启动子的CII依赖性激活中的作用：重要残基的鉴定及α C末端结构域的定位
Nucleic Acids Res. 2004 Feb 3;32(2):834-41. doi: 10.1093/nar/gkh230. Print 2004.
3
Characterization of a third, cII-dependent, coordinately activated promoter on phage lambda involved in lysogenic development.对噬菌体λ上第三个依赖于cII且与溶源发育相关的协同激活启动子的表征。
J Biol Chem. 1985 Sep 25;260(21):11838-44.
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DNA sequence of regulatory region for integration gene of bacteriophage lambda.噬菌体λ整合基因调控区的DNA序列
Proc Natl Acad Sci U S A. 1980 May;77(5):2477-81. doi: 10.1073/pnas.77.5.2477.
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Kinetic analysis of mutations affecting the cII activation site at the PRE promoter of bacteriophage lambda.噬菌体λ PRE 启动子上影响 cII 激活位点的突变的动力学分析。
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6432-6. doi: 10.1073/pnas.81.20.6432.
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Purified lambda regulatory protein cII positively activates promoters for lysogenic development.纯化的λ调节蛋白cII可正向激活溶原性发育的启动子。
Nature. 1981 Jul 9;292(5819):128-32. doi: 10.1038/292128a0.
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Involvement of the Escherichia coli RNA polymerase alpha subunit in transcriptional activation by the bacteriophage lambda CI and CII proteins.大肠杆菌RNA聚合酶α亚基参与噬菌体λ CI和CII蛋白的转录激活。
Gene. 1992 Dec 1;122(1):1-7. doi: 10.1016/0378-1119(92)90025-k.
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Interactions among CII protein, RNA polymerase and the lambda PRE promoter: contacts between RNA polymerase and the -35 region of PRE are identical in the presence and absence of CII protein.CII蛋白、RNA聚合酶与λ PRE启动子之间的相互作用：无论有无CII蛋白，RNA聚合酶与PRE的 -35区域之间的接触都是相同的。
Nucleic Acids Res. 2004 Feb 10;32(3):1083-90. doi: 10.1093/nar/gkh261. Print 2004.
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Role of cII protein in stimulating transcription initiation at the lambda PRE promoter. Enhanced formation and stabilization of open complexes.cII蛋白在刺激λ PRE启动子转录起始中的作用。开放复合物形成和稳定性的增强。
J Mol Biol. 1984 Feb 5;172(4):489-506. doi: 10.1016/s0022-2836(84)80019-4.
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Mutations that alter the DNA binding site for the bacteriophage lambda cII protein and affect the translation efficiency of the cII gene.改变噬菌体λ cII蛋白DNA结合位点并影响cII基因翻译效率的突变。
J Mol Biol. 1984 Dec 25;180(4):865-80. doi: 10.1016/0022-2836(84)90261-4.

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