Yokomuro K, Miyahara S, Takahashi H, Kimura Y
Eur J Immunol. 1983 Nov;13(11):883-9. doi: 10.1002/eji.1830131105.
The lymph node cells (LNC) activated in vivo by liver regeneration following partial hepatectomy of mice (primed lymph node cells) respond to regenerating liver cells in vitro with typical secondary immune response characteristics (Miyahara, S. et al., Eur. J. Immunol. 1983. 13: 878). These LNC activated in vivo suppress the proliferation of responder lymphocytes cultured with mitomycin C-treated regenerating syngeneic liver cells (sMLHLR). The suppressive activity was already present in LNC 4 days after partial hepatectomy and remained unchanged for at least 16 days. These primed LNC were effective not only on sMLHLR but also on syngeneic mixed lymphocyte culture (sMLR) and allogeneic mixed lymphocyte culture, of which responder cells share the I-A (I-B) subregions of the major histocompatibility complex (MHC) with primed LNC. At least one cell in the suppressor circuit is a T cell. The primed LNC restimulates in vitro with regenerating liver cells (in vitro reactivated primed LNC) suppressed the proliferation of syngeneic responder cells in sMLR, but not of cells from congeneic mice differing from the in vitro reactivated primed LNC at a cluster of genes linked to the Ig locus. Thus the suppressive activity of primed LNC is controlled by the I-A (I-B) subregions of the MHC and that of in vitro reactivated primed LNC by genes in the Ig region. The role of these suppressive cells in liver regeneration is discussed.
小鼠部分肝切除术后肝脏再生在体内激活的淋巴结细胞（LNC，致敏淋巴结细胞）在体外对再生肝细胞产生具有典型二次免疫应答特征的反应（宫原，S. 等人，《欧洲免疫学杂志》，1983年。13: 878）。这些在体内被激活的LNC抑制了用丝裂霉素C处理的同基因再生肝细胞培养的反应性淋巴细胞的增殖（sMLHLR）。部分肝切除术后4天，LNC中就已存在抑制活性，并且至少16天保持不变。这些致敏LNC不仅对sMLHLR有效，而且对同基因混合淋巴细胞培养（sMLR）和异基因混合淋巴细胞培养也有效，其中反应细胞与致敏LNC共享主要组织相容性复合体（MHC）的I-A（I-B）亚区。抑制回路中的至少一种细胞是T细胞。用再生肝细胞在体外再次刺激的致敏LNC（体外再激活的致敏LNC）抑制了sMLR中同基因反应细胞的增殖，但对来自与体外再激活的致敏LNC在与Ig基因座连锁的一组基因上不同的同类系小鼠的细胞没有抑制作用。因此，致敏LNC的抑制活性由MHC的I-A（I-B）亚区控制，而体外再激活的致敏LNC的抑制活性由Ig区域的基因控制。讨论了这些抑制细胞在肝脏再生中的作用。
