Miyahara S, Yokomuro K, Takahashi H, Kimura Y
Eur J Immunol. 1983 Nov;13(11):878-83. doi: 10.1002/eji.1830131104.
When syngeneic lymphocytes and mitomycin C-treated regenerating liver cells, prepared from partially hepatectomized mice, are cultured together, the in vitro DNA synthesis is activated (sMLHLR: syngeneic mixed hepatectomized-liver cell-lymphocyte culture). The Ia+ Kupffer cells play an important role as stimulators in the response, since the stimulating activity of regenerating liver cells is lost either by the pretreatment with anti-Ia monoclonal antibody plus complement or by the removing Kupffer cells from them. The lymphocytes are also activated in vivo during liver regeneration following partial hepatectomy. When lymphocytes, prepared from hepatectomized mice, are cultured with regenerating liver cells, the lymphocytes are stimulated to accelerate their DNA synthesis in a manner typical of the secondary immune responses (secondary sMLHLR). In primary sMLHLR, the responder cells are mainly Lyt-1+, whereas, in secondary sMLHLR, they are mainly Lyt-2+. The mechanism of changing the Lyt phenotype of major responder cells from Lyt-1 to Lyt-2 during sMLHLR is discussed.
将同基因淋巴细胞与经丝裂霉素C处理的、取自部分肝切除小鼠的再生肝细胞共同培养时，体外DNA合成被激活（sMLHLR：同基因混合肝切除肝细胞-淋巴细胞培养）。Ia⁺库普弗细胞作为刺激物在该反应中起重要作用，因为再生肝细胞的刺激活性可因用抗Ia单克隆抗体加补体预处理或从其中去除库普弗细胞而丧失。在部分肝切除后的肝再生过程中，淋巴细胞在体内也被激活。当将取自肝切除小鼠的淋巴细胞与再生肝细胞共同培养时，淋巴细胞受到刺激，以典型的二次免疫反应方式加速其DNA合成（二次sMLHLR）。在初次sMLHLR中，应答细胞主要是Lyt-1⁺，而在二次sMLHLR中，它们主要是Lyt-2⁺。本文讨论了在sMLHLR过程中主要应答细胞的Lyt表型从Lyt-1转变为Lyt-2的机制。
