Miyahara S
Nihon Geka Gakkai Zasshi. 1984 Aug;85(8):791-8.
When syngeneic lymphocytes and mitomycin C (MMC) treated regenerating liver cells prepared from a partial hepatectomized mice are cultured together, the in vitro DNA synthetic response is activated (sMLHLR: syngeneic mixed hepatectomized liver cell-lymphocyte culture). Ia+ Kupffer cells play an important role as stimulators in the responses, since the stimulating activity of regenerating liver cells is lost by the pretreatment of them with anti-Ia monoclonal antibody plus complement or the removing Kupffer cells from them. The lymphocytes are activated also in vivo during liver regeneration after a partial hepatectomy. Because, when lymphocytes prepared from hepatectomized mice are cultured with regenerating liver cells, lymphocytes are stimulated to accelerate their DNA synthesis in a typical manner of the secondary immune responses (secondary sMLHLR). In primary sMLHLR, the responder cells are mainly Lyt-1+ whereas in secondary sMLHLR they are mainly Lyt-2+. The mechanism of changing the Lyt phenotype of major responder cells from Lyt-1 to Lyt-2 during sMLHLR is discussed.
将同基因淋巴细胞与经丝裂霉素C（MMC）处理的、取自部分肝切除小鼠的再生肝细胞共同培养时，体外DNA合成反应被激活（sMLHLR：同基因混合肝切除肝细胞-淋巴细胞培养）。Ia⁺库普弗细胞在该反应中作为刺激细胞发挥重要作用，因为用抗Ia单克隆抗体加补体预处理再生肝细胞或从其中去除库普弗细胞后，其刺激活性丧失。在部分肝切除后的肝再生过程中，淋巴细胞在体内也被激活。因为，当取自肝切除小鼠的淋巴细胞与再生肝细胞共同培养时，淋巴细胞会受到刺激，以典型的二次免疫反应方式加速其DNA合成（二次sMLHLR）。在初次sMLHLR中，反应细胞主要是Lyt-1⁺，而在二次sMLHLR中它们主要是Lyt-2⁺。本文讨论了在sMLHLR过程中主要反应细胞的Lyt表型从Lyt-1转变为Lyt-2的机制。
