Martin L N, Gormus B J, Bozelka B E
Cell Immunol. 1983 Apr 15;77(2):338-47. doi: 10.1016/0008-8749(83)90034-5.
The percentages of rhesus monkey blood lymphocytes (PBL) reactive with OKT4 and OKT8 antibodies and the OKT4/OKT8 ratio showed significant correlations with the log of the immunoglobulin plaque-forming cell (PFC) response after stimulation with pokeweed mitogen (PWM). These correlations suggested that monkey OKT4+ cells function as "helper" cells and OKT8+ cells function as "suppressor" cells for the PFC response. This was confirmed by separation and study of enriched T- and B-cell subpopulations. OKT8-depleted (OKT4+) and OKT4-depleted (OKT8+) cells were obtained by treatment of purified T cells with antibody and complement. OKT4+ cells augmented the PWM-induced B-cell differentiation into PFC but OKT8+ cells did not. OKT8+ cells suppressed the PFC response by mixtures of B cells and OKT4+ cells. OKT8 antibodies also detected a suppressive cell subset in African green monkeys since the percentage of OKT8+ cells showed a negative correlation with the log PFC response. OKT4 antibodies failed to bind to African green monkey PBL.
与OKT4和OKT8抗体发生反应的恒河猴血淋巴细胞（PBL）的百分比以及OKT4/OKT8比值，与用美洲商陆丝裂原（PWM）刺激后的免疫球蛋白空斑形成细胞（PFC）反应的对数呈显著相关性。这些相关性表明，对于PFC反应，猴OKT4 +细胞起“辅助”细胞的作用，而OKT8 +细胞起“抑制”细胞的作用。通过对富集的T细胞和B细胞亚群的分离和研究证实了这一点。用抗体和补体处理纯化的T细胞，得到OKT8缺失（OKT4 +）和OKT4缺失（OKT8 +）的细胞。OKT4 +细胞增强了PWM诱导的B细胞分化为PFC，但OKT8 +细胞则不然。OKT8 +细胞抑制了B细胞和OKT4 +细胞混合物的PFC反应。OKT8抗体也在非洲绿猴中检测到一个抑制细胞亚群，因为OKT8 +细胞的百分比与PFC反应对数呈负相关。OKT4抗体未能与非洲绿猴PBL结合。
