• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过DNase I足迹法揭示放线菌素D与DNA的结合。

Binding of actinomycin D to DNA revealed by DNase I footprinting.

作者信息

Scamrov A V, Beabealashvilli R S

出版信息

FEBS Lett. 1983 Nov 28;164(1):97-101. doi: 10.1016/0014-5793(83)80027-1.

DOI:10.1016/0014-5793(83)80027-1
PMID:6228440
Abstract

We have analyzed the specificity of the actinomycin D-DNA interaction. The 'footprint' method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X not equal to G and Y not equal to C in accordance with RNA elongation hindrance sites [1]. (iii) There is a positive cooperativity in binding of actinomycin D with DNA.

摘要

我们分析了放线菌素D与DNA相互作用的特异性。本研究采用了“足迹”法。结果表明：（i）单个药物分子的结合需要二核苷酸GC或GG的存在。（ii）强结合位点由四核苷酸XGCY编码；根据RNA延伸阻碍位点[1]，其中X不等于G且Y不等于C。（iii）放线菌素D与DNA的结合存在正协同性。

相似文献

1
Binding of actinomycin D to DNA revealed by DNase I footprinting.通过DNase I足迹法揭示放线菌素D与DNA的结合。
FEBS Lett. 1983 Nov 28;164(1):97-101. doi: 10.1016/0014-5793(83)80027-1.
2
Methidiumpropyl-EDTA.Fe(II) and DNase I footprinting report different small molecule binding site sizes on DNA.甲基丙基-乙二胺四乙酸铁（II）和脱氧核糖核酸酶I足迹法揭示了DNA上不同的小分子结合位点大小。
Nucleic Acids Res. 1983 Aug 25;11(16):5555-67. doi: 10.1093/nar/11.16.5555.
3
Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I.通过抗DNase I分析放线菌素D和纺锤菌素与pBR322 DNA结合的序列特异性
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3260-4. doi: 10.1073/pnas.80.11.3260.
4
Effect of actinomycin on a (TA)6 plasmid insert.放线菌素对（TA）6质粒插入片段的作用。
Anticancer Drug Des. 1990 Feb;5(1):89-92.
5
Secondary (non-GpC) binding sites for actinomycin on DNA.
Biochim Biophys Acta. 1992 Jul 15;1131(3):300-6. doi: 10.1016/0167-4781(92)90028-x.
6
Sequence-specific inhibition of RNA elongation by actinomycin D.放线菌素D对RNA延伸的序列特异性抑制
FEBS Lett. 1983 Aug 22;160(1-2):124-8. doi: 10.1016/0014-5793(83)80950-8.
7
Rate enhancements in the DNase I footprinting experiment.DNA酶I足迹实验中的速率增强。
Nucleic Acids Res. 1988 Feb 25;16(4):1359-69. doi: 10.1093/nar/16.4.1359.
8
DNase I footprinting of small molecule binding sites on DNA.DNA上小分子结合位点的DNA酶I足迹分析
Methods Mol Biol. 2005;288:319-42. doi: 10.1385/1-59259-823-4:319.
9
Visualising the kinetics of dissociation of actinomycin from individual sites in mixed sequence DNA by DNase I footprinting.通过DNA酶I足迹法可视化放线菌素从混合序列DNA中各个位点解离的动力学。
Nucleic Acids Res. 1993 Mar 25;21(6):1339-44. doi: 10.1093/nar/21.6.1339.
10
Site-specific binding constants for actinomycin D on DNA determined from footprinting studies.通过足迹分析研究确定的放线菌素D与DNA的位点特异性结合常数。
Biochemistry. 1992 Feb 4;31(4):1046-58. doi: 10.1021/bi00119a013.

引用本文的文献

1
Study of interactions between actinomycin D and oligonucleotides by microchip electrophoresis and ESI-MS.通过微芯片电泳和电喷雾电离质谱法研究放线菌素D与寡核苷酸之间的相互作用
Talanta. 2007 Apr 30;72(2):561-7. doi: 10.1016/j.talanta.2006.11.019. Epub 2006 Dec 21.
2
Topoisomerase I-mediated DNA cleavage induced by the minor groove-directed binding of bibenzimidazoles to a distal site.双苯并咪唑与远端位点的小沟定向结合诱导拓扑异构酶I介导的DNA切割。
J Mol Biol. 2007 Jan 19;365(3):561-9. doi: 10.1016/j.jmb.2006.10.032. Epub 2006 Oct 13.
3
Structure-affinity relationships for the binding of actinomycin D to DNA.
放线菌素D与DNA结合的结构-亲和力关系。
J Comput Aided Mol Des. 1997 Mar;11(2):114-28. doi: 10.1023/a:1008018106064.
4
Visualising the kinetics of dissociation of actinomycin from individual sites in mixed sequence DNA by DNase I footprinting.通过DNA酶I足迹法可视化放线菌素从混合序列DNA中各个位点解离的动力学。
Nucleic Acids Res. 1993 Mar 25;21(6):1339-44. doi: 10.1093/nar/21.6.1339.
5
Daunomycin modifies the sequence-selective recognition of DNA by actinomycin.柔红霉素改变了放线菌素对DNA的序列选择性识别。
Nucleic Acids Res. 1994 Dec 11;22(24):5241-6. doi: 10.1093/nar/22.24.5241.
6
DNA structural variations produced by actinomycin and distamycin as revealed by DNAase I footprinting.通过DNA酶I足迹法揭示的放线菌素和偏端霉素产生的DNA结构变异。
Nucleic Acids Res. 1984 Dec 21;12(24):9271-85. doi: 10.1093/nar/12.24.9271.
7
Sequence-specific binding of echinomycin to DNA: evidence for conformational changes affecting flanking sequences.放线菌素与DNA的序列特异性结合：影响侧翼序列构象变化的证据。
Nucleic Acids Res. 1984 Jun 25;12(12):4865-79. doi: 10.1093/nar/12.12.4865.
8
Stability of DNase I in footprinting experiments.足迹实验中脱氧核糖核酸酶I的稳定性
Nucleic Acids Res. 1988 Sep 12;16(17):8724. doi: 10.1093/nar/16.17.8724.
9
Rate enhancements in the DNase I footprinting experiment.DNA酶I足迹实验中的速率增强。
Nucleic Acids Res. 1988 Feb 25;16(4):1359-69. doi: 10.1093/nar/16.4.1359.
10
Binding of actinomycin D to DNA: evidence for a nonclassical high-affinity binding mode that does not require GpC sites.放线菌素D与DNA的结合：一种不需要GpC位点的非经典高亲和力结合模式的证据。
Proc Natl Acad Sci U S A. 1989 Jun;86(11):3968-72. doi: 10.1073/pnas.86.11.3968.