通过DNase I足迹法揭示放线菌素D与DNA的结合。

Binding of actinomycin D to DNA revealed by DNase I footprinting.

作者信息

Scamrov A V, Beabealashvilli R S

出版信息

FEBS Lett. 1983 Nov 28;164(1):97-101. doi: 10.1016/0014-5793(83)80027-1.

Abstract

We have analyzed the specificity of the actinomycin D-DNA interaction. The 'footprint' method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X not equal to G and Y not equal to C in accordance with RNA elongation hindrance sites [1]. (iii) There is a positive cooperativity in binding of actinomycin D with DNA.

摘要

我们分析了放线菌素D与DNA相互作用的特异性。本研究采用了“足迹”法。结果表明：（i）单个药物分子的结合需要二核苷酸GC或GG的存在。（ii）强结合位点由四核苷酸XGCY编码；根据RNA延伸阻碍位点[1]，其中X不等于G且Y不等于C。（iii）放线菌素D与DNA的结合存在正协同性。