Gutkowska J, Thibault G, Milne R W, Januszewicz P, Schiller P W, Cantin M, Genest J
Proc Soc Exp Biol Med. 1984 Jun;176(2):105-8. doi: 10.3181/00379727-176-2-rc1.
We describe a solid phase radioimmunoassay for atrial natriuretic factor (ANF) and its application for measurement of this peptide in homogenates of rat atria. The method uses a synthetic 26 amino-acid fragment (8-33 ANF) of the native peptide. Sample (or standard) are incubated with the rabbit anti-8-33 ANF antiserum in peptide (8-33 ANF)-coated wells. Then an excess of I125 goat anti-rabbit IgG is added. The radioactivity bound is directly proportional to the amount of ANF present. The concentration of immunoreactive ANF has been found to be about 4 times higher in the right atrium than in the left atrium of the rat.
