Doggett R S, Wood G S, Horning S, Levy R, Dorfman R F, Bindl J, Warnke R A
Am J Pathol. 1984 May;115(2):245-52.
Ninety-five diffuse large cell lymphomas in 89 patients were stained in cryostat sections with a panel of monoclonal antibodies. Lymphoma cells from 47 patients (53%) expressed either kappa or lambda light chains, usually in combination with mu heavy chains. Fifteen samples from 12 patients (14%) expressed two or more T-cell antigens and commonly expressed Ia antigens. Lymphoma cells from 10 of these patients uniformly lacked one or more pan T-cell antigens; lymphoma cells from 4 of these patients also lacked both T-subset antigens--findings which should prove useful in diagnosis. Lymphomas from 28 patients (31%) did not express immunoglobulin or T-cell antigens but commonly expressed the B-lineage antigen B1; and the remaining 9 cases generally expressed Ia antigens, common ALL antigens, or both. Our findings confirm the marked immunologic heterogeneity of diffuse large cell lymphomas; the phenotypic heterogeneity observed in T-cell cases in many instances is difficult to reconcile with current models of T-cell differentiation.
用一组单克隆抗体对89例患者的95个弥漫性大细胞淋巴瘤进行冷冻切片染色。47例患者（53%）的淋巴瘤细胞表达κ或λ轻链，通常与μ重链同时表达。12例患者的15个样本（14%）表达两种或更多种T细胞抗原，且通常表达Ia抗原。其中10例患者的淋巴瘤细胞一致缺乏一种或多种泛T细胞抗原；其中4例患者的淋巴瘤细胞还缺乏两种T亚群抗原——这些发现对诊断很有用。28例患者（31%）的淋巴瘤不表达免疫球蛋白或T细胞抗原，但通常表达B系抗原B1；其余9例通常表达Ia抗原、常见的急性淋巴细胞白血病抗原或两者都表达。我们的发现证实了弥漫性大细胞淋巴瘤存在明显的免疫异质性；在许多情况下，T细胞病例中观察到的表型异质性难以与当前的T细胞分化模型相协调。
