Song M K, Adham N F
Am J Physiol. 1978 Feb;234(2):E99-105. doi: 10.1152/ajpendo.1978.234.2.E99.
The supernatant of cytoplasmic extract of rat small intestine was mixed with 65Zn and subjected to Sephadex G-75 column chromatography. The activity of 65Zn was detected in four peaks with estimated 800, 10,000, 50,000-75,000 mol wt, and larger than 100,000 daltons. The zinc-binding ligand from the 800 mol wt peak was prostaglandin or prostaglandin-like as indicated by ultra-violet absorption spectrum, by absorption spectrum shift in alcoholic potassium hydroxide, by its effects on pulmonary vascular resistance, and by radioimmunoassay. Addition of prostaglandin E2 or prostaglandin extracted from rat small intestine with ethyl acetate into the lumen of intestinal sacs resulted in a two to threefold increase in the activity of 65Zn in the external medium after 15-20 min incubation. The absorption and tissue distribution of orally administered 65Zn were decreased by 60% in indomethacin-treated rats and increased 70% when ethyl acetate-extracted prostaglandin and radioactive zinc were given simultaneously. These data suggest that prostaglandin not only binds zinc, but also facilitates its transport across the intestinal mucosa.
将大鼠小肠细胞质提取物的上清液与65锌混合，并进行Sephadex G - 75柱色谱分析。在四个峰中检测到65锌的活性，其估计分子量分别为800、10000、50000 - 75000道尔顿，以及大于100000道尔顿。通过紫外吸收光谱、在氢氧化钾乙醇溶液中的吸收光谱变化、对肺血管阻力的影响以及放射免疫测定表明，来自800道尔顿分子量峰的锌结合配体是前列腺素或类前列腺素。将前列腺素E2或用乙酸乙酯从大鼠小肠中提取的前列腺素添加到肠囊腔中，孵育15 - 20分钟后，外部介质中65锌的活性增加了两到三倍。在吲哚美辛处理的大鼠中，口服65锌的吸收和组织分布降低了60%，而当同时给予乙酸乙酯提取的前列腺素和放射性锌时，吸收和组织分布增加了70%。这些数据表明，前列腺素不仅能结合锌，还能促进其跨肠黏膜的转运。
