在Cu2+存在的情况下，抗坏血酸和丙糖还原酮对λDNA的片段化作用。

lambda DNA-fragmenting actions of ascorbic acid and triose reductone in the presence of Cu2+.

作者信息

Shinohara K, So M, Nonaka M, Nishiyama K, Murakami H, Omura H

出版信息

J Nutr Sci Vitaminol (Tokyo). 1983 Dec;29(6):671-8. doi: 10.3177/jnsv.29.671.

DOI:10.3177/jnsv.29.671

Abstract

Lambda DNA-fragmenting actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ were studied. The mixture of AsA or TR and Cu2+ caused a marked fragmentation of lambda DNA (3.2 X 10(7) daltons) within the first 1 min of reaction. Further incubation resulted in accumulation of the most abundant species of fragmented lambda DNA having a molecular weight of 1.3 X 10(5) daltons. The mixture of AsA or TR and Cu2+ fragmented calf thymus DNA to produce the fragmented DNA of which 5'-OH terminal groups have a mixture of free OH groups and phosphodiester linkage. The mixture of AsA or TR and Cu2+ was also found to fragment lambda DNA to produce dCMP predominantly as 5'-OH terminal nucleotides.

摘要

研究了在Cu2+存在下抗坏血酸（AsA）和三糖还原酮（TR）对λDNA的片段化作用。AsA或TR与Cu2+的混合物在反应的最初1分钟内导致λDNA（3.2×10^7道尔顿）明显片段化。进一步孵育导致分子量为1.3×10^5道尔顿的最丰富的片段化λDNA物种积累。AsA或TR与Cu2+的混合物使小牛胸腺DNA片段化，产生5'-OH末端基团具有游离OH基团和磷酸二酯键混合物的片段化DNA。还发现AsA或TR与Cu2+的混合物使λDNA片段化，主要产生dCMP作为5'-OH末端核苷酸。